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A method to rapidly create protein aggregates in living cells

Author

Listed:
  • Yusuke Miyazaki

    (Department of Chemical & Systems Biology Stanford University)

  • Kota Mizumoto

    (Department of Biology Stanford University
    Present address: Department of Zoology, The University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3)

  • Gautam Dey

    (Department of Chemical & Systems Biology Stanford University
    Present address: UCL-MRC Lab for Molecular Cell Biology, London WC1E 6BT, UK.)

  • Takamasa Kudo

    (Department of Chemical & Systems Biology Stanford University)

  • John Perrino

    (Cell Sciences Imaging Facility Stanford University)

  • Ling-chun Chen

    (Department of Chemical & Systems Biology Stanford University)

  • Tobias Meyer

    (Department of Chemical & Systems Biology Stanford University)

  • Thomas J. Wandless

    (Department of Chemical & Systems Biology Stanford University)

Abstract

The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a chemically controllable fluorescent protein that enables us to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor the movement and coalescence of individual aggregates into larger structures. This method can be applied to diverse experimental systems, including live animals, and may prove valuable for understanding cellular responses and diseases associated with protein aggregates.

Suggested Citation

  • Yusuke Miyazaki & Kota Mizumoto & Gautam Dey & Takamasa Kudo & John Perrino & Ling-chun Chen & Tobias Meyer & Thomas J. Wandless, 2016. "A method to rapidly create protein aggregates in living cells," Nature Communications, Nature, vol. 7(1), pages 1-7, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11689
    DOI: 10.1038/ncomms11689
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    Cited by:

    1. Sukanta Jash & Sayani Banerjee & Shibin Cheng & Bin Wang & Chenxi Qiu & Asami Kondo & Jan Ernerudh & Xiao Zhen Zhou & Kun Ping Lu & Surendra Sharma, 2023. "Cis P-tau is a central circulating and placental etiologic driver and therapeutic target of preeclampsia," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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