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Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping

Author

Listed:
  • Per Augustsson

    (Massachusetts Institute of Technology
    Lund University)

  • Jonas T. Karlsen

    (Technical University of Denmark)

  • Hao-Wei Su

    (Massachusetts Institute of Technology)

  • Henrik Bruus

    (Technical University of Denmark)

  • Joel Voldman

    (Massachusetts Institute of Technology)

Abstract

Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells’ interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a cell-friendly medium, and use acoustic forces to maintain that gradient in the presence of destabilizing forces. Applying this method we demonstrate iso-acoustic focusing of cell lines and leukocytes, showing that acoustic properties provide phenotypic information independent of size.

Suggested Citation

  • Per Augustsson & Jonas T. Karlsen & Hao-Wei Su & Henrik Bruus & Joel Voldman, 2016. "Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping," Nature Communications, Nature, vol. 7(1), pages 1-9, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11556
    DOI: 10.1038/ncomms11556
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    Cited by:

    1. Ye Yang & Yaozhang Yang & Dingyuan Liu & Yuanyuan Wang & Minqiao Lu & Qi Zhang & Jiqing Huang & Yongchuan Li & Teng Ma & Fei Yan & Hairong Zheng, 2023. "In-vivo programmable acoustic manipulation of genetically engineered bacteria," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Liang Shen & Zhenhua Tian & Kaichun Yang & Joseph Rich & Jianping Xia & Neil Upreti & Jinxin Zhang & Chuyi Chen & Nanjing Hao & Zhichao Pei & Tony Jun Huang, 2024. "Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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