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The elongation factor Spt5 facilitates transcription initiation for rapid induction of inflammatory-response genes

Author

Listed:
  • Gil Diamant

    (The Weizmann Institute of Science)

  • Anat Bahat

    (The Weizmann Institute of Science)

  • Rivka Dikstein

    (The Weizmann Institute of Science)

Abstract

A subset of inflammatory-response NF-κB target genes is activated immediately following pro-inflammatory signal. Here we followed the kinetics of primary transcript accumulation after NF-κB activation when the elongation factor Spt5 is knocked down. While elongation rate is unchanged, the transcript synthesis at the 5′-end and at the earliest time points is delayed and reduced, suggesting an unexpected role in early transcription. Investigating the underlying mechanism reveals that the induced TFIID–promoter association is practically abolished by Spt5 depletion. This effect is associated with a decrease in promoter-proximal H3K4me3 and H4K5Ac histone modifications that are differentially required for rapid transcriptional induction. In contrast, the displacement of TFIIE and Mediator, which occurs during promoter escape, is attenuated in the absence of Spt5. Our findings are consistent with a central role of Spt5 in maintenance of TFIID–promoter association and promoter escape to support rapid transcriptional induction and re-initiation of inflammatory-response genes.

Suggested Citation

  • Gil Diamant & Anat Bahat & Rivka Dikstein, 2016. "The elongation factor Spt5 facilitates transcription initiation for rapid induction of inflammatory-response genes," Nature Communications, Nature, vol. 7(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11547
    DOI: 10.1038/ncomms11547
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    Cited by:

    1. Krzysztof Kuś & Loic Carrique & Tea Kecman & Marjorie Fournier & Sarah Sayed Hassanein & Ebru Aydin & Cornelia Kilchert & Jonathan M. Grimes & Lidia Vasiljeva, 2025. "DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts," Nature Communications, Nature, vol. 16(1), pages 1-19, December.

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