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Generation of stem cell-derived β-cells from patients with type 1 diabetes

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  • Jeffrey R. Millman

    (Metabolism and Lipid Research, Washington University School of Medicine, Campus Box 8127, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Washington University in St Louis, 1 Brookings Drive, St Louis, Missouri 63130, USA)

  • Chunhui Xie

    (Harvard Stem Cell Institute, Harvard University)

  • Alana Van Dervort

    (Case Western Reserve University School of Medicine)

  • Mads Gürtler

    (Harvard Stem Cell Institute, Harvard University)

  • Felicia W. Pagliuca

    (Semma Therapeutics, Inc.)

  • Douglas A. Melton

    (Harvard Stem Cell Institute, Harvard University)

Abstract

We recently reported the scalable in vitro production of functional stem cell-derived β-cells (SC-β cells). Here we extend this approach to generate the first SC-β cells from type 1 diabetic patients (T1D). β-cells are destroyed during T1D disease progression, making it difficult to extensively study them in the past. These T1D SC-β cells express β-cell markers, respond to glucose both in vitro and in vivo, prevent alloxan-induced diabetes in mice and respond to anti-diabetic drugs. Furthermore, we use an in vitro disease model to demonstrate the cells respond to different forms of β-cell stress. Using these assays, we find no major differences in T1D SC-β cells compared with SC-β cells derived from non-diabetic patients. These results show that T1D SC-β cells could potentially be used for the treatment of diabetes, drug screening and the study of β-cell biology.

Suggested Citation

  • Jeffrey R. Millman & Chunhui Xie & Alana Van Dervort & Mads Gürtler & Felicia W. Pagliuca & Douglas A. Melton, 2016. "Generation of stem cell-derived β-cells from patients with type 1 diabetes," Nature Communications, Nature, vol. 7(1), pages 1-9, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11463
    DOI: 10.1038/ncomms11463
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    Cited by:

    1. Marlie M. Maestas & Matthew Ishahak & Punn Augsornworawat & Daniel A. Veronese-Paniagua & Kristina G. Maxwell & Leonardo Velazco-Cruz & Erica Marquez & Jiameng Sun & Mira Shunkarova & Sarah E. Gale & , 2024. "Identification of unique cell type responses in pancreatic islets to stress," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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