IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms11364.html
   My bibliography  Save this article

CRL4Wdr70 regulates H2B monoubiquitination and facilitates Exo1-dependent resection

Author

Listed:
  • Ming Zeng

    (Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University)

  • Laifeng Ren

    (Fenyang College of Shanxi Medical University)

  • Ken'Ichi Mizuno

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Konstantinos Nestoras

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Haibin Wang

    (Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University)

  • Zizhi Tang

    (Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University)

  • Liandi Guo

    (Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University)

  • Daochun Kong

    (National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University)

  • Qiwen Hu

    (College of Basic Medical Sciences, Third Military Medical University)

  • Qun He

    (State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University)

  • Lilin Du

    (National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park)

  • Antony M. Carr

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Cong Liu

    (Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University)

Abstract

Double-strand breaks repaired by homologous recombination (HR) are first resected to form single-stranded DNA, which binds replication protein A (RPA). RPA attracts mediators that load the Rad51 filament to promote strand invasion, the defining feature of HR. How the resection machinery navigates nucleosome-packaged DNA is poorly understood. Here we report that in Schizosaccharomyces pombe a conserved DDB1-CUL4-associated factor (DCAF), Wdr70, is recruited to DSBs as part of the Cullin4-DDB1 ubiquitin ligase (CRL4Wdr70) and stimulates distal H2B lysine 119 mono-ubiquitination (uH2B). Wdr70 deletion, or uH2B loss, results in increased loading of the checkpoint adaptor and resection inhibitor Crb253BP1, decreased Exo1 association and delayed resection. Wdr70 is dispensable for resection upon Crb253BP1 loss, or when the Set9 methyltransferase that creates docking sites for Crb2 is deleted. Finally, we establish that this histone regulatory cascade similarly controls DSB resection in human cells.

Suggested Citation

  • Ming Zeng & Laifeng Ren & Ken'Ichi Mizuno & Konstantinos Nestoras & Haibin Wang & Zizhi Tang & Liandi Guo & Daochun Kong & Qiwen Hu & Qun He & Lilin Du & Antony M. Carr & Cong Liu, 2016. "CRL4Wdr70 regulates H2B monoubiquitination and facilitates Exo1-dependent resection," Nature Communications, Nature, vol. 7(1), pages 1-11, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11364
    DOI: 10.1038/ncomms11364
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms11364
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms11364?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11364. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.