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The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules

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  • Kenneth A. Halberg

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Davidson Building Room 324, Glasgow G12 8QQ, UK
    Section for Cell & Neurobiology, University of Copenhagen)

  • Stephanie M. Rainey

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Davidson Building Room 324, Glasgow G12 8QQ, UK
    MRC—University of Glasgow Centre for Virus Research)

  • Iben R. Veland

    (Cancer Research UK | Beatson Institute, Garscube Estate
    Section of Cell Biology and Physiology, University of Copenhagen)

  • Helen Neuert

    (Institut für Neuro- und Verhaltensbiologie, Universität Münster)

  • Anthony J. Dornan

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Davidson Building Room 324, Glasgow G12 8QQ, UK)

  • Christian Klämbt

    (Institut für Neuro- und Verhaltensbiologie, Universität Münster)

  • Shireen-Anne Davies

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Davidson Building Room 324, Glasgow G12 8QQ, UK)

  • Julian A. T. Dow

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Davidson Building Room 324, Glasgow G12 8QQ, UK)

Abstract

Multicellular organisms rely on cell adhesion molecules to coordinate cell–cell interactions, and to provide navigational cues during tissue formation. In Drosophila, Fasciclin 2 (Fas2) has been intensively studied due to its role in nervous system development and maintenance; yet, Fas2 is most abundantly expressed in the adult renal (Malpighian) tubule rather than in neuronal tissues. The role Fas2 serves in this epithelium is unknown. Here we show that Fas2 is essential to brush border maintenance in renal tubules of Drosophila. Fas2 is dynamically expressed during tubule morphogenesis, localizing to the brush border whenever the tissue is transport competent. Genetic manipulations of Fas2 expression levels impact on both microvilli length and organization, which in turn dramatically affect stimulated rates of fluid secretion by the tissue. Consequently, we demonstrate a radically different role for this well-known cell adhesion molecule, and propose that Fas2-mediated intermicrovillar homophilic adhesion complexes help stabilize the brush border.

Suggested Citation

  • Kenneth A. Halberg & Stephanie M. Rainey & Iben R. Veland & Helen Neuert & Anthony J. Dornan & Christian Klämbt & Shireen-Anne Davies & Julian A. T. Dow, 2016. "The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules," Nature Communications, Nature, vol. 7(1), pages 1-10, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11266
    DOI: 10.1038/ncomms11266
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