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A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor

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  • Camille Sayou

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17
    Present address: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh EH9 3BF, UK)

  • Max H. Nanao

    (European Molecular Biology Laboratory)

  • Marc Jamin

    (Institut de Biologie Structurale CEA/DRF, CNRS, Université Grenoble Alpes, 71, avenue des Martyrs)

  • David Posé

    (Max Planck Institute for Developmental Biology
    Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Facultad de Ciencias, Universidad de Málaga)

  • Emmanuel Thévenon

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17)

  • Laura Grégoire

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17)

  • Gabrielle Tichtinsky

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17)

  • Grégoire Denay

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17)

  • Felix Ott

    (Max Planck Institute for Developmental Biology
    Present address: CeGaT GmbH, 72076 Tübingen, Germany)

  • Marta Peirats Llobet

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17
    Present address: Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas–Universidad Politecnica de Valencia, ES–46022 Valencia, Spain)

  • Markus Schmid

    (Max Planck Institute for Developmental Biology
    Umeå Plant Science Centre, Umeå University)

  • Renaud Dumas

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17)

  • François Parcy

    (Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS UMR5168, CEA/DRF/BIG, INRA UMR 1417, 17
    Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, University of British Columbia)

Abstract

Deciphering the mechanisms directing transcription factors (TFs) to specific genome regions is essential to understand and predict transcriptional regulation. TFs recognize short DNA motifs primarily through their DNA-binding domain. Some TFs also possess an oligomerization domain suspected to potentiate DNA binding but for which the genome-wide influence remains poorly understood. Here we focus on the LEAFY transcription factor, a master regulator of flower development in angiosperms. We have determined the crystal structure of its conserved amino-terminal domain, revealing an unanticipated Sterile Alpha Motif oligomerization domain. We show that this domain is essential to LEAFY floral function. Moreover, combined biochemical and genome-wide assays suggest that oligomerization is required for LEAFY to access regions with low-affinity binding sites or closed chromatin. This finding shows that domains that do not directly contact DNA can nevertheless have a profound impact on the DNA binding landscape of a TF.

Suggested Citation

  • Camille Sayou & Max H. Nanao & Marc Jamin & David Posé & Emmanuel Thévenon & Laura Grégoire & Gabrielle Tichtinsky & Grégoire Denay & Felix Ott & Marta Peirats Llobet & Markus Schmid & Renaud Dumas & , 2016. "A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor," Nature Communications, Nature, vol. 7(1), pages 1-12, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11222
    DOI: 10.1038/ncomms11222
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