IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms11166.html
   My bibliography  Save this article

Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening

Author

Listed:
  • Kerstin Klinkert

    (Institut Pasteur
    Centre National de la Recherche Scientifique UMR3691
    Sorbonne Universités, Université Pierre et Marie Curie, Université Paris 06, Institut de formation doctorale)

  • Murielle Rocancourt

    (Institut Pasteur
    Centre National de la Recherche Scientifique UMR3691)

  • Anne Houdusse

    (Institut Curie, Structural Motility Lab)

  • Arnaud Echard

    (Institut Pasteur
    Centre National de la Recherche Scientifique UMR3691)

Abstract

Establishment and maintenance of apico-basal polarity in epithelial organs must be tightly coupled with cell division, but the underlying molecular mechanisms are largely unknown. Using 3D cultures of renal MDCK cells (cysts), we found that the Rab35 GTPase plays a crucial role in polarity initiation and apical lumen positioning during the first cell division of cyst development. At the molecular level, Rab35 physically couples cytokinesis with the initiation of apico-basal polarity by tethering intracellular vesicles containing key apical determinants at the cleavage site. These vesicles transport aPKC, Cdc42, Crumbs3 and the lumen-promoting factor Podocalyxin, and are tethered through a direct interaction between Rab35 and the cytoplasmic tail of Podocalyxin. Consequently, Rab35 inactivation leads to complete inversion of apico-basal polarity in 3D cysts. This novel and unconventional mode of Rab-dependent vesicle targeting provides a simple mechanism for triggering both initiation of apico-basal polarity and lumen opening at the centre of cysts.

Suggested Citation

  • Kerstin Klinkert & Murielle Rocancourt & Anne Houdusse & Arnaud Echard, 2016. "Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening," Nature Communications, Nature, vol. 7(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11166
    DOI: 10.1038/ncomms11166
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms11166
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms11166?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Sara Bisi & Stefano Marchesi & Abrar Rizvi & Davide Carra & Galina V. Beznoussenko & Ines Ferrara & Gianluca Deflorian & Alexander Mironov & Giovanni Bertalot & Federica Pisati & Amanda Oldani & Angel, 2020. "IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules," Nature Communications, Nature, vol. 11(1), pages 1-23, December.
    2. Caitlin R. Francis & Hayle Kincross & Erich J. Kushner, 2022. "Rab35 governs apicobasal polarity through regulation of actin dynamics during sprouting angiogenesis," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11166. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.