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Diverse human extracellular RNAs are widely detected in human plasma

Author

Listed:
  • Jane E. Freedman

    (University of Massachusetts Medical School)

  • Mark Gerstein

    (Yale University Medical School, Computational Biology and Bioinformatics Program)

  • Eric Mick

    (University of Massachusetts Medical School)

  • Joel Rozowsky

    (Yale University Medical School, Computational Biology and Bioinformatics Program)

  • Daniel Levy

    (The Framingham Heart Study
    Population Sciences Branch, National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Robert Kitchen

    (Yale University Medical School, Computational Biology and Bioinformatics Program)

  • Saumya Das

    (Cardiovascular Institute, Beth Israel Deaconess Medical Center)

  • Ravi Shah

    (Cardiovascular Institute, Beth Israel Deaconess Medical Center)

  • Kirsty Danielson

    (Cardiovascular Institute, Beth Israel Deaconess Medical Center)

  • Lea Beaulieu

    (University of Massachusetts Medical School)

  • Fabio C. P. Navarro

    (Yale University Medical School, Computational Biology and Bioinformatics Program)

  • Yaoyu Wang

    (Center for Cancer Computational Biology, Dana Farber Cancer Institute)

  • Timur R. Galeev

    (Yale University Medical School, Computational Biology and Bioinformatics Program)

  • Alex Holman

    (Center for Cancer Computational Biology, Dana Farber Cancer Institute)

  • Raymond Y. Kwong

    (Brigham and Women's Hospital)

  • Venkatesh Murthy

    (University of Michigan)

  • Selim E. Tanriverdi

    (University of Massachusetts Medical School)

  • Milka Koupenova

    (University of Massachusetts Medical School)

  • Ekaterina Mikhalev

    (University of Massachusetts Medical School)

  • Kahraman Tanriverdi

    (University of Massachusetts Medical School)

Abstract

There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population.

Suggested Citation

  • Jane E. Freedman & Mark Gerstein & Eric Mick & Joel Rozowsky & Daniel Levy & Robert Kitchen & Saumya Das & Ravi Shah & Kirsty Danielson & Lea Beaulieu & Fabio C. P. Navarro & Yaoyu Wang & Timur R. Gal, 2016. "Diverse human extracellular RNAs are widely detected in human plasma," Nature Communications, Nature, vol. 7(1), pages 1-14, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11106
    DOI: 10.1038/ncomms11106
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    Cited by:

    1. Chunyan Li & Qiuyue Liu & Xiangyu Wang & Wenping Hu & Deping Han & Joram Mwashigadi Mwacharo & Caihong Wei & Mingxing Chu & Ran Di, 2020. "Expression and localization of PIWI proteins in testis and ovary of domestic sheep," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 65(3), pages 86-96.
    2. Jun Wang & Jinyong Huang & Yunlong Hu & Qianwen Guo & Shasha Zhang & Jinglin Tian & Yanqin Niu & Ling Ji & Yuzhong Xu & Peijun Tang & Yaqin He & Yuna Wang & Shuya Zhang & Hao Yang & Kang Kang & Xinchu, 2024. "Terminal modifications independent cell-free RNA sequencing enables sensitive early cancer detection and classification," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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