IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms11095.html
   My bibliography  Save this article

Identification of plant vacuolar transporters mediating phosphate storage

Author

Listed:
  • Tzu-Yin Liu

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan
    Present address: Department of Life Science and Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 30013, Taiwan.)

  • Teng-Kuei Huang

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan
    Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica
    Graduate Institute of Biotechnology, National Chung-Hsing University)

  • Shu-Yi Yang

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Yu-Ting Hong

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Sheng-Min Huang

    (National Tsing Hua University)

  • Fu-Nien Wang

    (National Tsing Hua University)

  • Su-Fen Chiang

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Shang-Yueh Tsai

    (Graduate Institute of Applied Physics, National Chengchi University)

  • Wen-Chien Lu

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Tzyy-Jen Chiou

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan
    Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica
    Biotechnology Center, National Chung-Hsing University)

Abstract

Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on 31P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth.

Suggested Citation

  • Tzu-Yin Liu & Teng-Kuei Huang & Shu-Yi Yang & Yu-Ting Hong & Sheng-Min Huang & Fu-Nien Wang & Su-Fen Chiang & Shang-Yueh Tsai & Wen-Chien Lu & Tzyy-Jen Chiou, 2016. "Identification of plant vacuolar transporters mediating phosphate storage," Nature Communications, Nature, vol. 7(1), pages 1-11, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11095
    DOI: 10.1038/ncomms11095
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms11095
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms11095?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Mushtak Kisko & Vishnu Shukla & Mandeep Kaur & Nadia Bouain & Nanthana Chaiwong & Benoit Lacombe & Ajay Kumar Pandey & Hatem Rouached, 2018. "Phosphorus Transport in Arabidopsis and Wheat: Emerging Strategies to Improve P Pool in Seeds," Agriculture, MDPI, vol. 8(2), pages 1-12, February.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11095. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.