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Identification of plant vacuolar transporters mediating phosphate storage

Author

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  • Tzu-Yin Liu

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan
    Present address: Department of Life Science and Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 30013, Taiwan.)

  • Teng-Kuei Huang

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan
    Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica
    Graduate Institute of Biotechnology, National Chung-Hsing University)

  • Shu-Yi Yang

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Yu-Ting Hong

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Sheng-Min Huang

    (National Tsing Hua University)

  • Fu-Nien Wang

    (National Tsing Hua University)

  • Su-Fen Chiang

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Shang-Yueh Tsai

    (Graduate Institute of Applied Physics, National Chengchi University)

  • Wen-Chien Lu

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan)

  • Tzyy-Jen Chiou

    (Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan
    Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica
    Biotechnology Center, National Chung-Hsing University)

Abstract

Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on 31P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth.

Suggested Citation

  • Tzu-Yin Liu & Teng-Kuei Huang & Shu-Yi Yang & Yu-Ting Hong & Sheng-Min Huang & Fu-Nien Wang & Su-Fen Chiang & Shang-Yueh Tsai & Wen-Chien Lu & Tzyy-Jen Chiou, 2016. "Identification of plant vacuolar transporters mediating phosphate storage," Nature Communications, Nature, vol. 7(1), pages 1-11, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11095
    DOI: 10.1038/ncomms11095
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    Cited by:

    1. Mushtak Kisko & Vishnu Shukla & Mandeep Kaur & Nadia Bouain & Nanthana Chaiwong & Benoit Lacombe & Ajay Kumar Pandey & Hatem Rouached, 2018. "Phosphorus Transport in Arabidopsis and Wheat: Emerging Strategies to Improve P Pool in Seeds," Agriculture, MDPI, vol. 8(2), pages 1-12, February.
    2. Can Liu & Zhen Bai & Yu Luo & Yanfeng Zhang & Yongfeng Wang & Hexin Liu & Meng Luo & Xiaofang Huang & Anle Chen & Lige Ma & Chen Chen & Jinwei Yuan & Ying Xu & Yantao Zhu & Jianxin Mu & Ran An & Cuili, 2024. "Multiomics dissection of Brassica napus L. lateral roots and endophytes interactions under phosphorus starvation," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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