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Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension

Author

Listed:
  • Natalie K. Lee

    (Queensland Brain Institute, The University of Queensland)

  • Ka Wai Fok

    (Queensland Brain Institute, The University of Queensland)

  • Amanda White

    (Queensland Brain Institute, The University of Queensland)

  • Nicole H. Wilson

    (Queensland Brain Institute, The University of Queensland)

  • Conor J. O’Leary

    (Queensland Brain Institute, The University of Queensland)

  • Hayley L. Cox

    (Queensland Brain Institute, The University of Queensland
    Present address: Princess Alexandra Hospital, 199 Ipswich Road, Woolloongabba, Brisbane, Queensland 4102, Australia)

  • Magdalene Michael

    (Institute for Molecular Bioscience, The University of Queensland)

  • Alpha S. Yap

    (Institute for Molecular Bioscience, The University of Queensland)

  • Helen M. Cooper

    (Queensland Brain Institute, The University of Queensland)

Abstract

To maintain tissue integrity during epithelial morphogenesis, adherens junctions (AJs) must resist the mechanical stresses exerted by dynamic tissue movements. Junctional stability is dependent on actomyosin contractility within the actin ring. Here we describe a novel function for the axon guidance receptor, Neogenin, as a key component of the actin nucleation machinery governing junctional stability. Loss of Neogenin perturbs AJs and attenuates junctional tension. Neogenin promotes actin nucleation at AJs by recruiting the Wave regulatory complex (WRC) and Arp2/3. A direct interaction between the Neogenin WIRS domain and the WRC is crucial for the spatially restricted recruitment of the WRC to the junction. Thus, we provide the first example of a functional WIRS–WRC interaction in epithelia. We further show that Neogenin regulates cadherin recycling at the AJ. In summary, we identify Neogenin as a pivotal component of the AJ, where it influences both cadherin dynamics and junctional tension.

Suggested Citation

  • Natalie K. Lee & Ka Wai Fok & Amanda White & Nicole H. Wilson & Conor J. O’Leary & Hayley L. Cox & Magdalene Michael & Alpha S. Yap & Helen M. Cooper, 2016. "Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension," Nature Communications, Nature, vol. 7(1), pages 1-13, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11082
    DOI: 10.1038/ncomms11082
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