IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms11047.html
   My bibliography  Save this article

Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness

Author

Listed:
  • Elizabeth Hinde

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Elvis Pandžić

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Zhengmin Yang

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Ivan H. W. Ng

    (Program in Emerging Infectious Diseases, Duke-NUS Medical School
    Nuclear Signalling Lab, Faculty of Biomedical and Psychological Sciences, Monash University)

  • David A. Jans

    (Nuclear Signalling Lab, Faculty of Biomedical and Psychological Sciences, Monash University)

  • Marie A. Bogoyevitch

    (Cell Signalling Research Laboratories, University of Melbourne)

  • Enrico Gratton

    (Laboratory for Fluorescence Dynamics, University of California)

  • Katharina Gaus

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

Abstract

Oligomerization of transcription factors controls their translocation into the nucleus and DNA-binding activity. Here we present a fluorescence microscopy analysis termed pCOMB (pair correlation of molecular brightness) that tracks the mobility of different oligomeric species within live cell nuclear architecture. pCOMB amplifies the signal from the brightest species present and filters the dynamics of the extracted oligomeric population based on arrival time between two locations. We use this method to demonstrate a dependence of signal transducer and activator of transcription 3 (STAT3) mobility on oligomeric state. We find that on entering the nucleus STAT3 dimers must first bind DNA to form STAT3 tetramers, which are also DNA-bound but exhibit a different mobility signature. Examining the dimer-to-tetramer transition by a cross-pair correlation analysis (cpCOMB) reveals that chromatin accessibility modulates STAT3 tetramer formation. Thus, the pCOMB approach is suitable for mapping the impact oligomerization on transcription factor dynamics.

Suggested Citation

  • Elizabeth Hinde & Elvis Pandžić & Zhengmin Yang & Ivan H. W. Ng & David A. Jans & Marie A. Bogoyevitch & Enrico Gratton & Katharina Gaus, 2016. "Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11047
    DOI: 10.1038/ncomms11047
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms11047
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms11047?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Falk Schneider & Pablo F. Cespedes & Narain Karedla & Michael L. Dustin & Marco Fritzsche, 2024. "Quantifying biomolecular organisation in membranes with brightness-transit statistics," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11047. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.