IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms11026.html
   My bibliography  Save this article

Multiplexed single-molecule force spectroscopy using a centrifuge

Author

Listed:
  • Darren Yang

    (School of engineering and applied sciences, Harvard University)

  • Andrew Ward

    (Program in Cellular and Molecular Medicine, Boston Children’s Hospital
    Harvard Medical School)

  • Ken Halvorsen

    (Program in Cellular and Molecular Medicine, Boston Children’s Hospital
    Harvard Medical School
    The RNA Institute, University at Albany)

  • Wesley P. Wong

    (Program in Cellular and Molecular Medicine, Boston Children’s Hospital
    Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

Abstract

We present a miniature centrifuge force microscope (CFM) that repurposes a benchtop centrifuge for high-throughput single-molecule experiments with high-resolution particle tracking, a large force range, temperature control and simple push-button operation. Incorporating DNA nanoswitches to enable repeated interrogation by force of single molecular pairs, we demonstrate increased throughput, reliability and the ability to characterize population heterogeneity. We perform spatiotemporally multiplexed experiments to collect 1,863 bond rupture statistics from 538 traceable molecular pairs in a single experiment, and show that 2 populations of DNA zippers can be distinguished using per-molecule statistics to reduce noise.

Suggested Citation

  • Darren Yang & Andrew Ward & Ken Halvorsen & Wesley P. Wong, 2016. "Multiplexed single-molecule force spectroscopy using a centrifuge," Nature Communications, Nature, vol. 7(1), pages 1-7, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11026
    DOI: 10.1038/ncomms11026
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms11026
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms11026?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jibin Abraham Punnoose & Kevin J. Thomas & Arun Richard Chandrasekaran & Javier Vilcapoma & Andrew Hayden & Kacey Kilpatrick & Sweta Vangaveti & Alan Chen & Thomas Banco & Ken Halvorsen, 2023. "High-throughput single-molecule quantification of individual base stacking energies in nucleic acids," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Ehsan Akbari & Melika Shahhosseini & Ariel Robbins & Michael G. Poirier & Jonathan W. Song & Carlos E. Castro, 2022. "Low cost and massively parallel force spectroscopy with fluid loading on a chip," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11026. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.