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Actin nucleation at the centrosome controls lymphocyte polarity

Author

Listed:
  • Dorian Obino

    (INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University)

  • Francesca Farina

    (CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes)

  • Odile Malbec

    (INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University)

  • Pablo J. Sáez

    (INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University)

  • Mathieu Maurin

    (INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University)

  • Jérémie Gaillard

    (CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes)

  • Florent Dingli

    (Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University)

  • Damarys Loew

    (Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University)

  • Alexis Gautreau

    (Ecole Polytechnique, CNRS UMR7654)

  • Maria-Isabel Yuseff

    (Pontificia Universidad Catolica de Chile)

  • Laurent Blanchoin

    (CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes)

  • Manuel Théry

    (CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes
    CytoMorpho Lab, Hopital Saint Louis, Institut Universitaire d'Hematologie, UMRS1160, CEA/INSERM/Université Paris Diderot)

  • Ana-Maria Lennon-Duménil

    (INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University)

Abstract

Cell polarity is required for the functional specialization of many cell types including lymphocytes. A hallmark of cell polarity is the reorientation of the centrosome that allows repositioning of organelles and vesicles in an asymmetric fashion. The mechanisms underlying centrosome polarization are not fully understood. Here we found that in resting lymphocytes, centrosome-associated Arp2/3 locally nucleates F-actin, which is needed for centrosome tethering to the nucleus via the LINC complex. Upon lymphocyte activation, Arp2/3 is partially depleted from the centrosome as a result of its recruitment to the immune synapse. This leads to a reduction in F-actin nucleation at the centrosome and thereby allows its detachment from the nucleus and polarization to the synapse. Therefore, F-actin nucleation at the centrosome—regulated by the availability of the Arp2/3 complex—determines its capacity to polarize in response to external stimuli.

Suggested Citation

  • Dorian Obino & Francesca Farina & Odile Malbec & Pablo J. Sáez & Mathieu Maurin & Jérémie Gaillard & Florent Dingli & Damarys Loew & Alexis Gautreau & Maria-Isabel Yuseff & Laurent Blanchoin & Manuel , 2016. "Actin nucleation at the centrosome controls lymphocyte polarity," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10969
    DOI: 10.1038/ncomms10969
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    Cited by:

    1. Muqing Cao & Xiaoxiao Zou & Chaoyi Li & Zaisheng Lin & Ni Wang & Zhongju Zou & Youqiong Ye & Joachim Seemann & Beth Levine & Zaiming Tang & Qing Zhong, 2023. "An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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