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Social amoebae trap and kill bacteria by casting DNA nets

Author

Listed:
  • Xuezhi Zhang

    (Science II, University of Geneva)

  • Olga Zhuchenko

    (Baylor College of Medicine, One Baylor Plaza)

  • Adam Kuspa

    (Baylor College of Medicine, One Baylor Plaza)

  • Thierry Soldati

    (Science II, University of Geneva)

Abstract

Extracellular traps (ETs) from neutrophils are reticulated nets of DNA decorated with anti-microbial granules, and are capable of trapping and killing extracellular pathogens. Various phagocytes of mammals and invertebrates produce ETs, however, the evolutionary history of this DNA-based host defence strategy is unclear. Here we report that Sentinel (S) cells of the multicellular slug stage of the social amoeba Dictyostelium discoideum produce ETs upon stimulation with bacteria or lipopolysaccharide in a reactive oxygen species-dependent manner. The production of ETs by S cells requires a Toll/Interleukin-1 receptor domain-containing protein TirA and reactive oxygen species-generating NADPH oxidases. Disruption of these genes results in decreased clearance of bacterial infections. Our results demonstrate that D. discoideum is a powerful model organism to study the evolution and conservation of mechanisms of cell-intrinsic immunity, and suggest that the origin of DNA-based ETs as an innate immune defence predates the emergence of metazoans.

Suggested Citation

  • Xuezhi Zhang & Olga Zhuchenko & Adam Kuspa & Thierry Soldati, 2016. "Social amoebae trap and kill bacteria by casting DNA nets," Nature Communications, Nature, vol. 7(1), pages 1-9, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10938
    DOI: 10.1038/ncomms10938
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    Cited by:

    1. Lauren E. Vandepas & Caroline Stefani & Phillip P. Domeier & Nikki Traylor-Knowles & Frederick W. Goetz & William E. Browne & Adam Lacy-Hulbert, 2024. "Extracellular DNA traps in a ctenophore demonstrate immune cell behaviors in a non-bilaterian," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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