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Nuclear PTEN functions as an essential regulator of SRF-dependent transcription to control smooth muscle differentiation

Author

Listed:
  • Henrick Horita

    (University of Colorado, Anschutz Medical Campus, 12700 East 19th Avenue, C281, Research Complex 2, Room 7101, Aurora, Colorado 80045, USA)

  • Christina L. Wysoczynski

    (University of Colorado, Anschutz Medical Campus)

  • Lori A. Walker

    (University of Colorado, Anschutz Medical Campus)

  • Karen S. Moulton

    (University of Colorado, Anschutz Medical Campus)

  • Marcella Li

    (University of Colorado, Anschutz Medical Campus)

  • Allison Ostriker

    (University of Colorado, Anschutz Medical Campus, 12700 East 19th Avenue, C281, Research Complex 2, Room 7101, Aurora, Colorado 80045, USA)

  • Rebecca Tucker

    (University of Colorado, Anschutz Medical Campus, 12700 East 19th Avenue, C281, Research Complex 2, Room 7101, Aurora, Colorado 80045, USA)

  • Timothy A. McKinsey

    (University of Colorado, Anschutz Medical Campus
    Center for Fibrosis Research and Translation, School of Medicine, University of Colorado, Anschutz Medical Campus)

  • Mair E. A. Churchill

    (University of Colorado, Anschutz Medical Campus)

  • Raphael A. Nemenoff

    (University of Colorado, Anschutz Medical Campus, 12700 East 19th Avenue, C281, Research Complex 2, Room 7101, Aurora, Colorado 80045, USA
    Cardiovascular Pulmonary Research Program, University of Colorado, Anschutz Medical Campus)

  • Mary C. M. Weiser-Evans

    (University of Colorado, Anschutz Medical Campus, 12700 East 19th Avenue, C281, Research Complex 2, Room 7101, Aurora, Colorado 80045, USA
    Center for Fibrosis Research and Translation, School of Medicine, University of Colorado, Anschutz Medical Campus
    Cardiovascular Pulmonary Research Program, University of Colorado, Anschutz Medical Campus)

Abstract

Vascular disease progression is associated with marked changes in vascular smooth muscle cell (SMC) phenotype and function. SMC contractile gene expression and, thus differentiation, is under direct transcriptional control by the transcription factor, serum response factor (SRF); however, the mechanisms dynamically regulating SMC phenotype are not fully defined. Here we report that the lipid and protein phosphatase, PTEN, has a novel role in the nucleus by functioning as an indispensible regulator with SRF to maintain the differentiated SM phenotype. PTEN interacts with the N-terminal domain of SRF and PTEN–SRF interaction promotes SRF binding to essential promoter elements in SM-specific genes. Factors inducing phenotypic switching promote loss of nuclear PTEN through nucleo-cytoplasmic translocation resulting in reduced myogenically active SRF, but enhanced SRF activity on target genes involved in proliferation. Overall decreased expression of PTEN was observed in intimal SMCs of human atherosclerotic lesions underlying the potential clinical importance of these findings.

Suggested Citation

  • Henrick Horita & Christina L. Wysoczynski & Lori A. Walker & Karen S. Moulton & Marcella Li & Allison Ostriker & Rebecca Tucker & Timothy A. McKinsey & Mair E. A. Churchill & Raphael A. Nemenoff & Mar, 2016. "Nuclear PTEN functions as an essential regulator of SRF-dependent transcription to control smooth muscle differentiation," Nature Communications, Nature, vol. 7(1), pages 1-17, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10830
    DOI: 10.1038/ncomms10830
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