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NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force

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  • Chenyu Li

    (Faculty of Science, National University of Singapore
    Present address: Harvard Medical School, Center for Life Science 0428, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA)

  • Chenyi Xue

    (Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania)

  • Qiaoyun Yang

    (Faculty of Science, National University of Singapore)

  • Boon Chuan Low

    (Faculty of Science, National University of Singapore
    Mechanobiology Institute, National University of Singapore)

  • Yih-Cherng Liou

    (Faculty of Science, National University of Singapore
    Graduate School for Integrative Sciences and Engineering, National University of Singapore)

Abstract

In vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and identify NuSAP as a novel regulator of the chromokinesin, Kid. Depletion of NuSAP significantly suppresses the amplitude and velocity of chromosome oscillation. We analyse the effects of NuSAP and Kid depletion in monopolar and bipolar cells with or without kinetochore microtubule depletion. Twelve postulated conditions are deciphered to reveal the contribution of NuSAP to the polar force generated at kinetochore microtubules and to the regulation of the polar ejection force generated by Kid, thus revealing a pivotal role of NuSAP in chromosome oscillation.

Suggested Citation

  • Chenyu Li & Chenyi Xue & Qiaoyun Yang & Boon Chuan Low & Yih-Cherng Liou, 2016. "NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10597
    DOI: 10.1038/ncomms10597
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