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MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation

Author

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  • Sophie Nolivos

    (University of Oxford)

  • Amy L. Upton

    (University of Oxford)

  • Anjana Badrinarayanan

    (University of Oxford
    Present address: Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA)

  • Julius Müller

    (The Jenner Institute, University of Oxford)

  • Katarzyna Zawadzka

    (University of Oxford)

  • Jakub Wiktor

    (University of Oxford
    Kavli Institute of NanoScience)

  • Amber Gill

    (University of Oxford)

  • Lidia Arciszewska

    (University of Oxford)

  • Emilien Nicolas

    (University of Oxford
    Present address: Laboratoire du Métabolisme de l’ARN FRS/FNRS-IBMM-CMMI, Université Libre de Bruxelles, Charleroi-Gosselies, B-6041 Belgium)

  • David Sherratt

    (University of Oxford)

Abstract

The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the replication termination region (ter). Consistent with this, MukBEF interacts with MatP, which binds matS sites in ter. MatP displaces wild-type MukBEF complexes from ter, thereby facilitating their association with ori, and limiting the availability of topoisomerase IV (TopoIV) at ter. Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. We propose that the interplay between MukBEF and MatP directs chromosome organization in relation to MukBEF clusters and associated topisomerase IV, thereby ensuring timely chromosome unlinking and segregation.

Suggested Citation

  • Sophie Nolivos & Amy L. Upton & Anjana Badrinarayanan & Julius Müller & Katarzyna Zawadzka & Jakub Wiktor & Amber Gill & Lidia Arciszewska & Emilien Nicolas & David Sherratt, 2016. "MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation," Nature Communications, Nature, vol. 7(1), pages 1-12, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10466
    DOI: 10.1038/ncomms10466
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    Cited by:

    1. Ismath Sadhir & Seán M. Murray, 2023. "Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Josh P. Prince & Jani R. Bolla & Gemma L. M. Fisher & Jarno Mäkelä & Marjorie Fournier & Carol V. Robinson & Lidia K. Arciszewska & David J. Sherratt, 2021. "Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation," Nature Communications, Nature, vol. 12(1), pages 1-12, December.

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