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Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing

Author

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  • Sanki Tashiro

    (Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan)

  • Tetsuya Handa

    (Graduate School of Science, Osaka University)

  • Atsushi Matsuda

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    Graduate School of Frontier Biosciences, Osaka University)

  • Takuto Ban

    (Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan)

  • Toru Takigawa

    (Graduate School of Science, Osaka University)

  • Kazumi Miyasato

    (Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan)

  • Kojiro Ishii

    (Graduate School of Frontier Biosciences, Osaka University)

  • Kazuto Kugou

    (The University of Tokyo
    Present address: Department of Frontier Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan)

  • Kunihiro Ohta

    (The University of Tokyo)

  • Yasushi Hiraoka

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    Graduate School of Frontier Biosciences, Osaka University)

  • Hisao Masukata

    (Graduate School of Science, Osaka University)

  • Junko Kanoh

    (Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan)

Abstract

A chromosome is composed of structurally and functionally distinct domains. However, the molecular mechanisms underlying the formation of chromatin structure and the function of subtelomeres, the telomere-adjacent regions, remain obscure. Here we report the roles of the conserved centromeric protein Shugoshin 2 (Sgo2) in defining chromatin structure and functions of the subtelomeres in the fission yeast Schizosaccharomyces pombe. We show that Sgo2 localizes at the subtelomeres preferentially during G2 phase and is essential for the formation of a highly condensed subtelomeric chromatin body ‘knob’. Furthermore, the absence of Sgo2 leads to the derepression of the subtelomeric genes and premature DNA replication at the subtelomeric late origins. Thus, the subtelomeric specialized chromatin domain organized by Sgo2 represses both transcription and replication to ensure proper gene expression and replication timing.

Suggested Citation

  • Sanki Tashiro & Tetsuya Handa & Atsushi Matsuda & Takuto Ban & Toru Takigawa & Kazumi Miyasato & Kojiro Ishii & Kazuto Kugou & Kunihiro Ohta & Yasushi Hiraoka & Hisao Masukata & Junko Kanoh, 2016. "Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing," Nature Communications, Nature, vol. 7(1), pages 1-12, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10393
    DOI: 10.1038/ncomms10393
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    Cited by:

    1. Javier Encinar del Dedo & M. Belén Suárez & Rafael López-San Segundo & Alicia Vázquez-Bolado & Jingjing Sun & Natalia García-Blanco & Patricia García & Pauline Tricquet & Jun-Song Chen & Peter C. Dedo, 2024. "The Greatwall-Endosulfine-PP2A/B55 pathway regulates entry into quiescence by enhancing translation of Elongator-tunable transcripts," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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