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Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects

Author

Listed:
  • Xiaojin Zhang

    (University of Michigan)

  • Yan Li

    (University of Michigan
    State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University)

  • Y. Eugene Chen

    (Frankel Cardiovascular Center, University of Michigan)

  • Jihua Chen

    (State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University)

  • Peter X. Ma

    (University of Michigan
    University of Michigan
    Macromolecular Science and Engineering Center, University of Michigan
    University of Michigan)

Abstract

MicroRNAs (miRNAs) are being developed to enhance tissue regeneration. Here we show that a hyperbranched polymer with high miRNA-binding affinity and negligible cytotoxicity can self-assemble into nano-sized polyplexes with a ‘double-shell’ miRNA distribution and high transfection efficiency. These polyplexes are encapsulated in biodegradable microspheres to enable controllable two-stage (polyplexes and miRNA) delivery. The microspheres are attached to cell-free nanofibrous polymer scaffolds that spatially control the release of miR-26a. This technology is used to regenerate critical-sized bone defects in osteoporotic mice by targeting Gsk-3β to activate the osteoblastic activity of endogenous stem cells, thus addressing a critical challenge in regenerative medicine of achieving cell-free scaffold-based miRNA therapy for tissue engineering.

Suggested Citation

  • Xiaojin Zhang & Yan Li & Y. Eugene Chen & Jihua Chen & Peter X. Ma, 2016. "Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects," Nature Communications, Nature, vol. 7(1), pages 1-15, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10376
    DOI: 10.1038/ncomms10376
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    Cited by:

    1. Gonggong Lu & Yang Xu & Quanying Liu & Manyu Chen & Huan Sun & Peilei Wang & Xing Li & Yuxiang Wang & Xiang Li & Xuhui Hui & En Luo & Jun Liu & Qing Jiang & Jie Liang & Yujiang Fan & Yong Sun & Xingdo, 2022. "An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis," Nature Communications, Nature, vol. 13(1), pages 1-20, December.

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