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A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth

Author

Listed:
  • Joseph H.A. Vissers

    (Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre
    University of Melbourne)

  • Samuel A. Manning

    (Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre
    University of Melbourne)

  • Aishwarya Kulkarni

    (Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre)

  • Kieran F. Harvey

    (Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre
    University of Melbourne
    University of Melbourne)

Abstract

Libraries of transgenic Drosophila melanogaster carrying RNA interference (RNAi) constructs have been used extensively to perform large-scale functional genetic screens in vivo. For example, RNAi screens have facilitated the discovery of multiple components of the Hippo pathway, an evolutionarily conserved growth-regulatory network. Here we investigate an important technical limitation with the widely used VDRC KK RNAi collection. We find that approximately 25% of VDRC KK RNAi lines cause false-positive enhancement of the Hippo pathway, owing to ectopic expression of the Tiptop transcription factor. Of relevance to the broader Drosophila community, ectopic tiptop (tio) expression can also cause organ malformations and mask phenotypes such as organ overgrowth. To enhance the use of the VDRC KK RNAi library, we have generated a D. melanogaster strain that will allow researchers to test, in a single cross, whether their genetic screen of interest will be affected by ectopic tio expression.

Suggested Citation

  • Joseph H.A. Vissers & Samuel A. Manning & Aishwarya Kulkarni & Kieran F. Harvey, 2016. "A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth," Nature Communications, Nature, vol. 7(1), pages 1-6, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10368
    DOI: 10.1038/ncomms10368
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    Cited by:

    1. Kenneth A. Wilson & Sudipta Bar & Eric B. Dammer & Enrique M. Carrera & Brian A. Hodge & Tyler A. U. Hilsabeck & Joanna Bons & George W. Brownridge & Jennifer N. Beck & Jacob Rose & Melia Granath-Pane, 2024. "OXR1 maintains the retromer to delay brain aging under dietary restriction," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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