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Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis

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  • Chunyan Wang

    (Rice University)

  • Jin H. Bae

    (Rice University)

  • David Yu Zhang

    (Rice University
    Systems, Synthetic, and Physical Biology, Rice University)

Abstract

DNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG° of DNA motifs at temperatures and buffer conditions of interest, with significantly better accuracy (6- to 14-fold lower s.e.) than prior methods. The equilibrium constant of a reaction with thermodynamics closely approximating that of a desired motif is numerically calculated from directly observed reactant and product equilibrium concentrations; a DNA catalyst is designed to accelerate equilibration. We measured the ΔG° of terminal fluorophores, single-nucleotide dangles and multinucleotide dangles, in temperatures ranging from 10 to 45 °C.

Suggested Citation

  • Chunyan Wang & Jin H. Bae & David Yu Zhang, 2016. "Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis," Nature Communications, Nature, vol. 7(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10319
    DOI: 10.1038/ncomms10319
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