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Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping

Author

Listed:
  • Kevin J. Freedman

    (Imperial College London)

  • Lauren M. Otto

    (University of Minnesota)

  • Aleksandar P. Ivanov

    (Imperial College London)

  • Avijit Barik

    (University of Minnesota
    University of Minnesota)

  • Sang-Hyun Oh

    (University of Minnesota
    University of Minnesota)

  • Joshua B. Edel

    (Imperial College London)

Abstract

Single-molecule techniques are being developed with the exciting prospect of revolutionizing the healthcare industry by generating vast amounts of genetic and proteomic data. One exceptionally promising route is in the use of nanopore sensors. However, a well-known complexity is that detection and capture is predominantly diffusion limited. This problem is compounded when taking into account the capture volume of a nanopore, typically 108–1010 times smaller than the sample volume. To rectify this disproportionate ratio, we demonstrate a simple, yet powerful, method based on coupling single-molecule dielectrophoretic trapping to nanopore sensing. We show that DNA can be captured from a controllable, but typically much larger, volume and concentrated at the tip of a metallic nanopore. This enables the detection of single molecules at concentrations as low as 5 fM, which is approximately a 103 reduction in the limit of detection compared with existing methods, while still maintaining efficient throughput.

Suggested Citation

  • Kevin J. Freedman & Lauren M. Otto & Aleksandar P. Ivanov & Avijit Barik & Sang-Hyun Oh & Joshua B. Edel, 2016. "Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping," Nature Communications, Nature, vol. 7(1), pages 1-9, April.
  • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10217
    DOI: 10.1038/ncomms10217
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    Cited by:

    1. Ren Ren & Shenglin Cai & Xiaona Fang & Xiaoyi Wang & Zheng Zhang & Micol Damiani & Charlotte Hudlerova & Annachiara Rosa & Joshua Hope & Nicola J. Cook & Peter Gorelkin & Alexander Erofeev & Pavel Nov, 2023. "Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Dan Zhang & Yongjie Sun & Zhichao Wang & Fang Liu & Xuanjun Zhang, 2023. "Switchable biomimetic nanochannels for on-demand SO2 detection by light-controlled photochromism," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Xiaojun Wei & Xiaoqin Wang & Zehui Zhang & Yuanyuan Luo & Zixin Wang & Wen Xiong & Piyush K. Jain & John R. Monnier & Hui Wang & Tony Y. Hu & Chuanbing Tang & Helmut Albrecht & Chang Liu, 2022. "A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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