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Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA

Author

Listed:
  • Walter F. Mangel

    (Brookhaven National Laboratory)

  • William J. McGrath

    (Brookhaven National Laboratory)

  • Kan Xiong

    (Massachusetts Institute of Technology
    Broad Institute of MIT and Harvard)

  • Vito Graziano

    (Brookhaven National Laboratory)

  • Paul C. Blainey

    (Massachusetts Institute of Technology
    Broad Institute of MIT and Harvard)

Abstract

Recently, we showed the adenovirus proteinase interacts productively with its protein substrates in vitro and in vivo in nascent virus particles via one-dimensional diffusion along the viral DNA. The mechanism by which this occurs has heretofore been unknown. We show sliding of these proteins along DNA occurs on a new vehicle in molecular biology, a ‘molecular sled’ named pVIc. This 11-amino acid viral peptide binds to DNA independent of sequence. pVIc slides on DNA, exhibiting the fastest one-dimensional diffusion constant, 26±1.8 × 106 (bp)2 s−1. pVIc is a ‘molecular sled,’ because it can slide heterologous cargos along DNA, for example, a streptavidin tetramer. Similar peptides, for example, from the C terminus of β-actin or NLSIII of the p53 protein, slide along DNA. Characteristics of the ‘molecular sled’ in its milieu (virion, nucleus) have implications for how proteins in the nucleus of cells interact and imply a new form of biochemistry, one-dimensional biochemistry.

Suggested Citation

  • Walter F. Mangel & William J. McGrath & Kan Xiong & Vito Graziano & Paul C. Blainey, 2016. "Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA," Nature Communications, Nature, vol. 7(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10202
    DOI: 10.1038/ncomms10202
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