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Confocal multiview light-sheet microscopy

Author

Listed:
  • Gustavo de Medeiros

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit)

  • Nils Norlin

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit
    European Molecular Biology Laboratory, Developmental Biology Unit)

  • Stefan Gunther

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit)

  • Marvin Albert

    (European Molecular Biology Laboratory, Developmental Biology Unit)

  • Laura Panavaite

    (European Molecular Biology Laboratory, Developmental Biology Unit)

  • Ulla-Maj Fiuza

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit)

  • Francesca Peri

    (European Molecular Biology Laboratory, Developmental Biology Unit)

  • Takashi Hiiragi

    (European Molecular Biology Laboratory, Developmental Biology Unit)

  • Uros Krzic

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit
    Present address: Carl Zeiss Microscopy Kistlerhofstrasse 75, 81379 Munich, Germany.)

  • Lars Hufnagel

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit)

Abstract

Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image contrast. Here we combine multiview light-sheet imaging with electronic confocal slit detection implemented on modern camera sensors. In addition to improved imaging quality, the electronic confocal slit detection doubles the acquisition speed in multiview setups with two opposing illumination directions allowing simultaneous dual-sided illumination. Confocal multiview light-sheet microscopy eliminates the need for specimen-specific data fusion algorithms, streamlines image post-processing, easing data handling and storage.

Suggested Citation

  • Gustavo de Medeiros & Nils Norlin & Stefan Gunther & Marvin Albert & Laura Panavaite & Ulla-Maj Fiuza & Francesca Peri & Takashi Hiiragi & Uros Krzic & Lars Hufnagel, 2015. "Confocal multiview light-sheet microscopy," Nature Communications, Nature, vol. 6(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9881
    DOI: 10.1038/ncomms9881
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    Cited by:

    1. Hannah J. Gustafson & Nikolas Claussen & Stefano Renzis & Sebastian J. Streichan, 2022. "Patterned mechanical feedback establishes a global myosin gradient," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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