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Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6

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  • Shigenori Miura

    (Institute of Industrial Science, The University of Tokyo
    Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST)
    Present address: Department of Cellular Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan)

  • Koji Sato

    (Institute of Industrial Science, The University of Tokyo
    Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST)
    Present address: Division of Bioregulatory Signaling, Okazaki Institute for Integrative Bioscience, 5-1 Higashiyama, Myodaijichou, Okazaki, Aichi 444-8585, Japan)

  • Midori Kato-Negishi

    (Institute of Industrial Science, The University of Tokyo
    Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST))

  • Tetsuhiko Teshima

    (Institute of Industrial Science, The University of Tokyo
    Present addresses: NTT Basic Research Laboratories, 3-1 Morinosato Wakamiya Atsugi-shi, Kanagawa 243-0198 Japan)

  • Shoji Takeuchi

    (Institute of Industrial Science, The University of Tokyo
    Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST))

Abstract

Microvilli are cellular membrane protrusions present on differentiated epithelial cells, which can sense and interact with the surrounding fluid environment. Biochemical and genetic approaches have identified a set of factors involved in microvilli formation; however, the underlying extrinsic regulatory mechanism of microvilli formation remains largely unknown. Here we demonstrate that fluid shear stress (FSS), an external mechanical cue, serves as a trigger for microvilli formation in human placental trophoblastic cells. We further reveal that the transient receptor potential, vanilloid family type-6 (TRPV6) calcium ion channel plays a critical role in flow-induced Ca2+ influx and microvilli formation. TRPV6 regulates phosphorylation of Ezrin via a Ca2+-dependent phosphorylation of Akt; this molecular event is necessary for microvillar localization of Ezrin in response to FSS. Our findings provide molecular insight into the microvilli-mediated mechanoresponsive cellular functions, such as epithelial absorption, signal perception and mechanotransduction.

Suggested Citation

  • Shigenori Miura & Koji Sato & Midori Kato-Negishi & Tetsuhiko Teshima & Shoji Takeuchi, 2015. "Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6," Nature Communications, Nature, vol. 6(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9871
    DOI: 10.1038/ncomms9871
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    Cited by:

    1. Takeshi Hori & Hiroaki Okae & Shun Shibata & Norio Kobayashi & Eri H. Kobayashi & Akira Oike & Asato Sekiya & Takahiro Arima & Hirokazu Kaji, 2024. "Trophoblast stem cell-based organoid models of the human placental barrier," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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