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Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture

Author

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  • Agla J. Fridriksdottir

    (Faculty of Health Sciences, University of Copenhagen
    Danish Stem Cell Centre, Faculty of Health Sciences, University of Copenhagen)

  • Jiyoung Kim

    (Faculty of Health Sciences, University of Copenhagen
    Danish Stem Cell Centre, Faculty of Health Sciences, University of Copenhagen)

  • René Villadsen

    (Faculty of Health Sciences, University of Copenhagen
    Danish Stem Cell Centre, Faculty of Health Sciences, University of Copenhagen)

  • Marie Christine Klitgaard

    (Faculty of Health Sciences, University of Copenhagen
    Danish Stem Cell Centre, Faculty of Health Sciences, University of Copenhagen
    University of Copenhagen)

  • Branden M. Hopkinson

    (Faculty of Health Sciences, University of Copenhagen
    Danish Stem Cell Centre, Faculty of Health Sciences, University of Copenhagen)

  • Ole William Petersen

    (Faculty of Health Sciences, University of Copenhagen
    Danish Stem Cell Centre, Faculty of Health Sciences, University of Copenhagen)

  • Lone Rønnov-Jessen

    (University of Copenhagen)

Abstract

Investigating the susceptibility of oestrogen receptor-positive (ERpos) normal human breast epithelial cells (HBECs) for clinical purposes or basic research awaits a proficient cell-based assay. Here we set out to identify markers for isolating ERpos cells and to expand what appear to be post-mitotic primary cells into exponentially growing cultures. We report a robust technique for isolating ERpos HBECs from reduction mammoplasties by FACS using two cell surface markers, CD166 and CD117, and an intracellular cytokeratin marker, Ks20.8, for further tracking single cells in culture. We show that ERpos HBECs are released from growth restraint by small molecule inhibitors of TGFβ signalling, and that growth is augmented further in response to oestrogen. Importantly, ER signalling is functionally active in ERpos cells in extended culture. These findings open a new avenue of experimentation with normal ERpos HBECs and provide a basis for understanding the evolution of human breast cancer.

Suggested Citation

  • Agla J. Fridriksdottir & Jiyoung Kim & René Villadsen & Marie Christine Klitgaard & Branden M. Hopkinson & Ole William Petersen & Lone Rønnov-Jessen, 2015. "Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture," Nature Communications, Nature, vol. 6(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9786
    DOI: 10.1038/ncomms9786
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    Cited by:

    1. Pauliina M. Munne & Lahja Martikainen & Iiris Räty & Kia Bertula & Nonappa & Janika Ruuska & Hanna Ala-Hongisto & Aino Peura & Babette Hollmann & Lilya Euro & Kerim Yavuz & Linda Patrikainen & Maria S, 2021. "Compressive stress-mediated p38 activation required for ERα + phenotype in breast cancer," Nature Communications, Nature, vol. 12(1), pages 1-17, December.

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