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An Xist-activating antisense RNA required for X-chromosome inactivation

Author

Listed:
  • Mrinal K. Sarkar

    (University of Michigan Medical School)

  • Srimonta Gayen

    (University of Michigan Medical School)

  • Surinder Kumar

    (University of Michigan Medical School)

  • Emily Maclary

    (University of Michigan Medical School)

  • Emily Buttigieg

    (University of Michigan Medical School)

  • Michael Hinten

    (University of Michigan Medical School)

  • Archana Kumari

    (University of Michigan Medical School)

  • Clair Harris

    (University of Michigan Medical School)

  • Takashi Sado

    (Graduate School of Agriculture, Kinki University)

  • Sundeep Kalantry

    (University of Michigan Medical School)

Abstract

The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.

Suggested Citation

  • Mrinal K. Sarkar & Srimonta Gayen & Surinder Kumar & Emily Maclary & Emily Buttigieg & Michael Hinten & Archana Kumari & Clair Harris & Takashi Sado & Sundeep Kalantry, 2015. "An Xist-activating antisense RNA required for X-chromosome inactivation," Nature Communications, Nature, vol. 6(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9564
    DOI: 10.1038/ncomms9564
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    Cited by:

    1. Milan Kumar Samanta & Srimonta Gayen & Clair Harris & Emily Maclary & Yumie Murata-Nakamura & Rebecca M. Malcore & Robert S. Porter & Patricia M. Garay & Christina N. Vallianatos & Paul B. Samollow & , 2022. "Activation of Xist by an evolutionarily conserved function of KDM5C demethylase," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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