IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms9535.html
   My bibliography  Save this article

Stonin1 mediates endocytosis of the proteoglycan NG2 and regulates focal adhesion dynamics and cell motility

Author

Listed:
  • Fabian Feutlinske

    (Leibniz-Institut für Molekulare Pharmakologie (FMP))

  • Marietta Browarski

    (Leibniz-Institut für Molekulare Pharmakologie (FMP))

  • Min-Chi Ku

    (Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine
    German Centre for Cardiovascular Research (DZHK))

  • Philipp Trnka

    (Leibniz-Institut für Molekulare Pharmakologie (FMP))

  • Sonia Waiczies

    (Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine
    German Centre for Cardiovascular Research (DZHK))

  • Thoralf Niendorf

    (Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine
    German Centre for Cardiovascular Research (DZHK))

  • William B. Stallcup

    (Tumor Microenvironment and Metastasis Program, Cancer Center, Sanford-Burnham Medical Research Institute)

  • Rainer Glass

    (Neurosurgical Research, Clinic for Neurosurgery, Ludwig Maximilians University of Munich)

  • Eberhard Krause

    (Leibniz-Institut für Molekulare Pharmakologie (FMP))

  • Tanja Maritzen

    (Leibniz-Institut für Molekulare Pharmakologie (FMP))

Abstract

Cellular functions, ranging from focal adhesion (FA) dynamics and cell motility to tumour growth, are orchestrated by signals cells receive from outside via cell surface receptors. Signalling is fine-tuned by the exo–endocytic cycling of these receptors to control cellular responses such as FA dynamics, which determine cell motility. How precisely endocytosis regulates turnover of the various cell surface receptors remains unclear. Here we identify Stonin1, an endocytic adaptor of unknown function, as a regulator of FA dynamics and cell motility, and demonstrate that it facilitates the internalization of the oncogenic proteoglycan NG2, a co-receptor of integrins and platelet-derived growth factor receptor. Embryonic fibroblasts obtained from Stonin1-deficient mice display a marked surface accumulation of NG2, increased cellular signalling and defective FA disassembly as well as altered cellular motility. These data establish Stonin1 as a specific adaptor for the endocytosis of NG2 and as an important factor for FA dynamics and cell migration.

Suggested Citation

  • Fabian Feutlinske & Marietta Browarski & Min-Chi Ku & Philipp Trnka & Sonia Waiczies & Thoralf Niendorf & William B. Stallcup & Rainer Glass & Eberhard Krause & Tanja Maritzen, 2015. "Stonin1 mediates endocytosis of the proteoglycan NG2 and regulates focal adhesion dynamics and cell motility," Nature Communications, Nature, vol. 6(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9535
    DOI: 10.1038/ncomms9535
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms9535
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms9535?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Fabian Lukas & Claudia Matthaeus & Tania López-Hernández & Ines Lahmann & Nicole Schultz & Martin Lehmann & Dmytro Puchkov & Jan Pielage & Volker Haucke & Tanja Maritzen, 2024. "Canonical and non-canonical integrin-based adhesions dynamically interconvert," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9535. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.