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SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Author

Listed:
  • Gražvydas Lukinavičius

    (Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology)

  • Claudia Blaukopf

    (Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC))

  • Elias Pershagen

    (Uppsala University)

  • Alberto Schena

    (Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology)

  • Luc Reymond

    (Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology)

  • Emmanuel Derivery

    (NCCR in Chemical Biology, University of Geneva)

  • Marcos Gonzalez-Gaitan

    (NCCR in Chemical Biology, University of Geneva)

  • Elisa D’Este

    (Max-Planck-Institute for Biophysical Chemistry)

  • Stefan W. Hell

    (Max-Planck-Institute for Biophysical Chemistry)

  • Daniel Wolfram Gerlich

    (Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC))

  • Kai Johnsson

    (Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology)

Abstract

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

Suggested Citation

  • Gražvydas Lukinavičius & Claudia Blaukopf & Elias Pershagen & Alberto Schena & Luc Reymond & Emmanuel Derivery & Marcos Gonzalez-Gaitan & Elisa D’Este & Stefan W. Hell & Daniel Wolfram Gerlich & Kai J, 2015. "SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy," Nature Communications, Nature, vol. 6(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9497
    DOI: 10.1038/ncomms9497
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