IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms9400.html
   My bibliography  Save this article

Wall mechanics and exocytosis define the shape of growth domains in fission yeast

Author

Listed:
  • Juan F. Abenza

    (University of Cambridge
    Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge)

  • Etienne Couturier

    (Universidad de Santiago de Chile)

  • James Dodgson

    (University of Cambridge
    Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge)

  • Johanna Dickmann

    (University of Cambridge
    Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge)

  • Anatole Chessel

    (University of Cambridge
    Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge)

  • Jacques Dumais

    (Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez
    Harvard University)

  • Rafael E. Carazo Salas

    (University of Cambridge
    Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge)

Abstract

The amazing structural variety of cells is matched only by their functional diversity, and reflects the complex interplay between biochemical and mechanical regulation. How both regulatory layers generate specifically shaped cellular domains is not fully understood. Here, we report how cell growth domains are shaped in fission yeast. Based on quantitative analysis of cell wall expansion and elasticity, we develop a model for how mechanics and cell wall assembly interact and use it to look for factors underpinning growth domain morphogenesis. Surprisingly, we find that neither the global cell shape regulators Cdc42-Scd1-Scd2 nor the major cell wall synthesis regulators Bgs1-Bgs4-Rgf1 are reliable predictors of growth domain geometry. Instead, their geometry can be defined by cell wall mechanics and the cortical localization pattern of the exocytic factors Sec6-Syb1-Exo70. Forceful re-directioning of exocytic vesicle fusion to broader cortical areas induces proportional shape changes to growth domains, demonstrating that both features are causally linked.

Suggested Citation

  • Juan F. Abenza & Etienne Couturier & James Dodgson & Johanna Dickmann & Anatole Chessel & Jacques Dumais & Rafael E. Carazo Salas, 2015. "Wall mechanics and exocytosis define the shape of growth domains in fission yeast," Nature Communications, Nature, vol. 6(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9400
    DOI: 10.1038/ncomms9400
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms9400
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms9400?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9400. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.