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NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation

Author

Listed:
  • Emi Murayama

    (Institut Pasteur
    CNRS, URA2578
    INSERM)

  • Milka Sarris

    (Institut Pasteur
    CNRS, URA2578
    Present address: University of Cambridge, Department of Physiology, Development and Neuroscience, Cambridge CB2 3DY, UK.)

  • Michael Redd

    (University of Utah, Huntsman Cancer Institute)

  • Dorothée Le Guyader

    (Institut Pasteur
    CNRS, URA2578)

  • Catherine Vivier

    (Institut Pasteur
    CNRS, URA2578)

  • Wyatt Horsley

    (University of Utah, Huntsman Cancer Institute)

  • Nikolaus Trede

    (University of Utah, Huntsman Cancer Institute)

  • Philippe Herbomel

    (Institut Pasteur
    CNRS, URA2578)

Abstract

The ontogeny of haematopoietic niches in vertebrates is essentially unknown. Here we show that the stromal cells of the caudal haematopoietic tissue (CHT), the first niche where definitive haematopoietic stem/progenitor cells (HSPCs) home in zebrafish development, derive from the caudal somites through an epithelial–mesenchymal transition (EMT). The resulting stromal cell progenitors accompany the formation of the caudal vein sinusoids, the other main component of the CHT niche, and mature into reticular cells lining and interconnecting sinusoids. We characterize a zebrafish mutant defective in definitive haematopoiesis due to a deficiency in the nascent polypeptide-associated complex alpha subunit (NACA). We demonstrate that the defect resides not in HSPCs but in the CHT niche. NACA-deficient stromal cell progenitors initially develop normally together with the sinusoids, and HSPCs home to the resulting niche, but stromal cell maturation is compromised, leading to a niche that is unable to support HSPC maintenance, expansion and differentiation.

Suggested Citation

  • Emi Murayama & Milka Sarris & Michael Redd & Dorothée Le Guyader & Catherine Vivier & Wyatt Horsley & Nikolaus Trede & Philippe Herbomel, 2015. "NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation," Nature Communications, Nature, vol. 6(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9375
    DOI: 10.1038/ncomms9375
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    Cited by:

    1. Takayuki Yamada & Yuto Yoshinari & Masayuki Tobo & Okiko Habara & Takashi Nishimura, 2023. "Nacα protects the larval fat body from cell death by maintaining cellular proteostasis in Drosophila," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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