IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms9314.html
   My bibliography  Save this article

How Leiomodin and Tropomodulin use a common fold for different actin assembly functions

Author

Listed:
  • Malgorzata Boczkowska

    (Perelman School of Medicine, University of Pennsylvania)

  • Grzegorz Rebowski

    (Perelman School of Medicine, University of Pennsylvania)

  • Elena Kremneva

    (Institute of Biotechnology, PO Box 56, 00014 University of Helsinki)

  • Pekka Lappalainen

    (Institute of Biotechnology, PO Box 56, 00014 University of Helsinki)

  • Roberto Dominguez

    (Perelman School of Medicine, University of Pennsylvania)

Abstract

How proteins sharing a common fold have evolved different functions is a fundamental question in biology. Tropomodulins (Tmods) are prototypical actin filament pointed-end-capping proteins, whereas their homologues, Leiomodins (Lmods), are powerful filament nucleators. We show that Tmods and Lmods do not compete biochemically, and display similar but distinct localization in sarcomeres. Changes along the polypeptide chains of Tmods and Lmods exquisitely adapt their functions for capping versus nucleation. Tmods have alternating tropomyosin (TM)- and actin-binding sites (TMBS1, ABS1, TMBS2 and ABS2). Lmods additionally contain a C-terminal extension featuring an actin-binding WH2 domain. Unexpectedly, the different activities of Tmods and Lmods do not arise from the Lmod-specific extension. Instead, nucleation by Lmods depends on two major adaptations—the loss of pointed-end-capping elements present in Tmods and the specialization of the highly conserved ABS2 for recruitment of two or more actin subunits. The WH2 domain plays only an auxiliary role in nucleation.

Suggested Citation

  • Malgorzata Boczkowska & Grzegorz Rebowski & Elena Kremneva & Pekka Lappalainen & Roberto Dominguez, 2015. "How Leiomodin and Tropomodulin use a common fold for different actin assembly functions," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9314
    DOI: 10.1038/ncomms9314
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms9314
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms9314?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9314. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.