IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms9307.html
   My bibliography  Save this article

Real-time fluorescence imaging with 20 nm axial resolution

Author

Listed:
  • Daniel R. Stabley

    (Emory University
    Present address: Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, Tennessee 38105, USA.)

  • Thomas Oh

    (Laboratory of Cellular Biophysics, The Rockefeller University)

  • Sanford M. Simon

    (Laboratory of Cellular Biophysics, The Rockefeller University)

  • Alexa L. Mattheyses

    (Emory University School of Medicine, Atlanta, Georgia 30322, USA)

  • Khalid Salaita

    (Emory University)

Abstract

Measuring the nanoscale organization of protein structures near the plasma membrane of live cells is challenging, especially when the structure is dynamic. Here we present the development of a two-wavelength total internal reflection fluorescence method capable of real-time imaging of cellular structure height with nanometre resolution. The method employs a protein of interest tagged with two different fluorophores and imaged to obtain the ratio of emission in the two channels. We use this approach to visualize the nanoscale organization of microtubules and endocytosis of the epidermal growth factor receptor.

Suggested Citation

  • Daniel R. Stabley & Thomas Oh & Sanford M. Simon & Alexa L. Mattheyses & Khalid Salaita, 2015. "Real-time fluorescence imaging with 20 nm axial resolution," Nature Communications, Nature, vol. 6(1), pages 1-7, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9307
    DOI: 10.1038/ncomms9307
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms9307
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms9307?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9307. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.