IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms9146.html
   My bibliography  Save this article

Chamber identity programs drive early functional partitioning of the heart

Author

Listed:
  • Christian Mosimann

    (Howard Hughes Medical Institute
    Stem Cell Program, Boston Children’s Hospital
    Boston Children’s Hospital, Harvard Stem Cell Institute, Harvard Medical School
    Institute of Molecular Life Sciences (IMLS), University of Zürich)

  • Daniela Panáková

    (Brigham and Women's Hospital, Harvard Medical School
    Present addresses: Max-Delbrück Center for Molecular Medicine (MDC), 13125 Berlin-Buch, Germany)

  • Andreas A. Werdich

    (Brigham and Women's Hospital, Harvard Medical School)

  • Gabriel Musso

    (Brigham and Women's Hospital, Harvard Medical School)

  • Alexa Burger

    (Institute of Molecular Life Sciences (IMLS), University of Zürich)

  • Katy L. Lawson

    (Howard Hughes Medical Institute
    Stem Cell Program, Boston Children’s Hospital
    Boston Children’s Hospital, Harvard Stem Cell Institute, Harvard Medical School)

  • Logan A. Carr

    (Howard Hughes Medical Institute
    Stem Cell Program, Boston Children’s Hospital
    Boston Children’s Hospital, Harvard Stem Cell Institute, Harvard Medical School)

  • Kathleen R. Nevis

    (Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School)

  • M. Khaled Sabeh

    (Brigham and Women's Hospital, Harvard Medical School)

  • Yi Zhou

    (Howard Hughes Medical Institute
    Stem Cell Program, Boston Children’s Hospital
    Boston Children’s Hospital, Harvard Stem Cell Institute, Harvard Medical School)

  • Alan J. Davidson

    (Howard Hughes Medical Institute
    Stem Cell Program, Boston Children’s Hospital
    Boston Children’s Hospital, Harvard Stem Cell Institute, Harvard Medical School)

  • Anthony DiBiase

    (Howard Hughes Medical Institute
    Stem Cell Program, Boston Children’s Hospital
    Boston Children’s Hospital, Harvard Stem Cell Institute, Harvard Medical School)

  • Caroline E. Burns

    (Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School)

  • C. Geoffrey Burns

    (Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School)

  • Calum A. MacRae

    (Brigham and Women's Hospital, Harvard Medical School)

  • Leonard I. Zon

    (Howard Hughes Medical Institute
    Stem Cell Program, Boston Children’s Hospital
    Boston Children’s Hospital, Harvard Stem Cell Institute, Harvard Medical School)

Abstract

The vertebrate heart muscle (myocardium) develops from the first heart field (FHF) and expands by adding second heart field (SHF) cells. While both lineages exist already in teleosts, the primordial contributions of FHF and SHF to heart structure and function remain incompletely understood. Here we delineate the functional contribution of the FHF and SHF to the zebrafish heart using the cis-regulatory elements of the draculin (drl) gene. The drl reporters initially delineate the lateral plate mesoderm, including heart progenitors. Subsequent myocardial drl reporter expression restricts to FHF descendants. We harnessed this unique feature to uncover that loss of tbx5a and pitx2 affect relative FHF versus SHF contributions to the heart. High-resolution physiology reveals distinctive electrical properties of each heart field territory that define a functional boundary within the single zebrafish ventricle. Our data establish that the transcriptional program driving cardiac septation regulates physiologic ventricle partitioning, which successively provides mechanical advantages of sequential contraction.

Suggested Citation

  • Christian Mosimann & Daniela Panáková & Andreas A. Werdich & Gabriel Musso & Alexa Burger & Katy L. Lawson & Logan A. Carr & Kathleen R. Nevis & M. Khaled Sabeh & Yi Zhou & Alan J. Davidson & Anthony , 2015. "Chamber identity programs drive early functional partitioning of the heart," Nature Communications, Nature, vol. 6(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9146
    DOI: 10.1038/ncomms9146
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms9146
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms9146?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Karin D. Prummel & Helena L. Crowell & Susan Nieuwenhuize & Eline C. Brombacher & Stephan Daetwyler & Charlotte Soneson & Jelena Kresoja-Rakic & Agnese Kocere & Manuel Ronner & Alexander Ernst & Zahra, 2022. "Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    2. Andrew P Holmes & Ting Y Yu & Samantha Tull & Fahima Syeda & Stefan M Kuhlmann & Sian-Marie O’Brien & Pushpa Patel & Keith L Brain & Davor Pavlovic & Nigel A Brown & Larissa Fabritz & Paulus Kirchhof, 2016. "A Regional Reduction in Ito and IKACh in the Murine Posterior Left Atrial Myocardium Is Associated with Action Potential Prolongation and Increased Ectopic Activity," PLOS ONE, Public Library of Science, vol. 11(5), pages 1-17, May.
    3. Shunya Kuroda & Robert L. Lalonde & Thomas A. Mansour & Christian Mosimann & Tetsuya Nakamura, 2024. "Multiple embryonic sources converge to form the pectoral girdle skeleton in zebrafish," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9146. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.