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A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation

Author

Listed:
  • Natasha L. Pirman

    (Yale University
    Systems Biology Institute, Yale University)

  • Karl W. Barber

    (Yale University
    Systems Biology Institute, Yale University)

  • Hans R. Aerni

    (Yale University
    Systems Biology Institute, Yale University)

  • Natalie J. Ma

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Adrian D. Haimovich

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Svetlana Rogulina

    (Yale University
    Systems Biology Institute, Yale University)

  • Farren J. Isaacs

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Jesse Rinehart

    (Yale University
    Systems Biology Institute, Yale University)

Abstract

Biochemical investigation of protein phosphorylation events is limited by inefficient production of the phosphorylated and non-phosphorylated forms of full-length proteins. Here using a genomically recoded strain of E. coli with a flexible UAG codon we produce site-specific serine- or phosphoserine-containing proteins, with purities approaching 90%, from a single recombinant DNA. Specifically, we synthesize human MEK1 kinase with two serines or two phosphoserines, from one DNA template, and demonstrate programmable kinase activity. Programmable protein phosphorylation is poised to help reveal the structural and functional information encoded in the phosphoproteome.

Suggested Citation

  • Natasha L. Pirman & Karl W. Barber & Hans R. Aerni & Natalie J. Ma & Adrian D. Haimovich & Svetlana Rogulina & Farren J. Isaacs & Jesse Rinehart, 2015. "A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation," Nature Communications, Nature, vol. 6(1), pages 1-6, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9130
    DOI: 10.1038/ncomms9130
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    Cited by:

    1. Jack M. Moen & Kyle Mohler & Svetlana Rogulina & Xiaojian Shi & Hongying Shen & Jesse Rinehart, 2022. "Enhanced access to the human phosphoproteome with genetically encoded phosphothreonine," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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