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High-resolution analysis of the human T-cell receptor repertoire

Author

Listed:
  • Eliana Ruggiero

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Jan P. Nicolay

    (German Cancer Research Center
    Venereology and Allergology, University Medical Center)

  • Raffaele Fronza

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Anne Arens

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Anna Paruzynski

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Ali Nowrouzi

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Gökçe Ürenden

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Christina Lulay

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Sven Schneider

    (Institute for Clinical Chemistry, University Medical Center, Ruprecht-Karls-University of Heidelberg)

  • Sergij Goerdt

    (Venereology and Allergology, University Medical Center)

  • Hanno Glimm

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Peter H. Krammer

    (German Cancer Research Center)

  • Manfred Schmidt

    (National Center for Tumor Diseases and German Cancer Research Center)

  • Christof von Kalle

    (National Center for Tumor Diseases and German Cancer Research Center)

Abstract

Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR α- and β-chain diversity without sequence-associated or quantitative restrictions in healthy and diseased conditions. TCR-LA-MC PCR identifies convergent recombination events, classifies different stages of cutaneous T-cell lymphoma in vivo and demonstrates TCR reactivation after in vitro cytomegalovirus stimulation. TCR-LA-MC PCR allows ultra-deep data access to both physiological TCR diversity and mechanisms influencing clonality in all clinical settings with restricted or distorted TCR repertoires.

Suggested Citation

  • Eliana Ruggiero & Jan P. Nicolay & Raffaele Fronza & Anne Arens & Anna Paruzynski & Ali Nowrouzi & Gökçe Ürenden & Christina Lulay & Sven Schneider & Sergij Goerdt & Hanno Glimm & Peter H. Krammer & M, 2015. "High-resolution analysis of the human T-cell receptor repertoire," Nature Communications, Nature, vol. 6(1), pages 1-7, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9081
    DOI: 10.1038/ncomms9081
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