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A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

Author

Listed:
  • Joon-Yung Cha

    (PMBBRC & IALS, Gyeongsang National University)

  • Woe-Yeon Kim

    (PMBBRC & IALS, Gyeongsang National University)

  • Sun Bin Kang

    (PMBBRC & IALS, Gyeongsang National University)

  • Jeong Im Kim

    (Purdue University)

  • Dongwon Baek

    (PMBBRC & IALS, Gyeongsang National University)

  • In Jung Jung

    (PMBBRC & IALS, Gyeongsang National University)

  • Mi Ri Kim

    (PMBBRC & IALS, Gyeongsang National University)

  • Ning Li

    (PMBBRC & IALS, Gyeongsang National University)

  • Hyun-Jin Kim

    (PMBBRC & IALS, Gyeongsang National University)

  • Masatoshi Nakajima

    (The University of Tokyo
    Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST))

  • Tadao Asami

    (The University of Tokyo
    Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
    King Abdulaziz University)

  • Jamal S. M. Sabir

    (Biotechnology Research Group, Faculty of Science, King Abdulaziz University)

  • Hyeong Cheol Park

    (National Institute of Ecology)

  • Sang Yeol Lee

    (PMBBRC & IALS, Gyeongsang National University)

  • Hans J. Bohnert

    (Biotechnology Research Group, Faculty of Science, King Abdulaziz University
    University of Illinois at Urbana-Champaign)

  • Ray A. Bressan

    (Biotechnology Research Group, Faculty of Science, King Abdulaziz University
    Purdue University)

  • Jose M. Pardo

    (Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas)

  • Dae-Jin Yun

    (PMBBRC & IALS, Gyeongsang National University)

Abstract

YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD- and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD- and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses.

Suggested Citation

  • Joon-Yung Cha & Woe-Yeon Kim & Sun Bin Kang & Jeong Im Kim & Dongwon Baek & In Jung Jung & Mi Ri Kim & Ning Li & Hyun-Jin Kim & Masatoshi Nakajima & Tadao Asami & Jamal S. M. Sabir & Hyeong Cheol Park, 2015. "A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis," Nature Communications, Nature, vol. 6(1), pages 1-13, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9041
    DOI: 10.1038/ncomms9041
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