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Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription

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  • Mihue Jang

    (Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu)

  • Jong Hwan Kim

    (Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu)

  • Hae Yun Nam

    (University of Ulsan College of Medicine)

  • Ick Chan Kwon

    (Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu)

  • Hyung Jun Ahn

    (Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu)

Abstract

For therapeutic applications of siRNA, there are technical challenges with respect to targeted and systemic delivery. We here report a new siRNA carrier, RNAtr NPs, in a way that multiple tandem copies of RNA hairpins as a result of rolling circle transcription (RCT) can be readily adapted in tumour-targeted and systemic siRNA delivery. RNAtr NPs provide a means of condensing large amounts of multimeric RNA transcripts into the compact nanoparticles, especially without the aid of polycationic agents, and thus reduce the risk of immunogenicity and cytotoxicity by avoiding the use of synthetic polycationic reagents. This strategy allows the design of a platform technology for systemic delivery of siRNA to tumour sites, because RCT reaction, which enzymatically generates RNA polymers in multiple copy numbers at low cost, can lead to directly accessible routes to targeted and systemic delivery. Therefore, RNAtr NPs suggest great potentials as the siRNA therapeutics for cancer treatment.

Suggested Citation

  • Mihue Jang & Jong Hwan Kim & Hae Yun Nam & Ick Chan Kwon & Hyung Jun Ahn, 2015. "Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8930
    DOI: 10.1038/ncomms8930
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    Cited by:

    1. Gerardo Patiño-Guillén & Jovan Pešović & Marko Panić & Dušanka Savić-Pavićević & Filip Bošković & Ulrich Felix Keyser, 2024. "Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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