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Whole-central nervous system functional imaging in larval Drosophila

Author

Listed:
  • William C. Lemon

    (Howard Hughes Medical Institute, Janelia Research Campus)

  • Stefan R. Pulver

    (Howard Hughes Medical Institute, Janelia Research Campus)

  • Burkhard Höckendorf

    (Howard Hughes Medical Institute, Janelia Research Campus)

  • Katie McDole

    (Howard Hughes Medical Institute, Janelia Research Campus)

  • Kristin Branson

    (Howard Hughes Medical Institute, Janelia Research Campus)

  • Jeremy Freeman

    (Howard Hughes Medical Institute, Janelia Research Campus)

  • Philipp J. Keller

    (Howard Hughes Medical Institute, Janelia Research Campus)

Abstract

Understanding how the brain works in tight concert with the rest of the central nervous system (CNS) hinges upon knowledge of coordinated activity patterns across the whole CNS. We present a method for measuring activity in an entire, non-transparent CNS with high spatiotemporal resolution. We combine a light-sheet microscope capable of simultaneous multi-view imaging at volumetric speeds 25-fold faster than the state-of-the-art, a whole-CNS imaging assay for the isolated Drosophila larval CNS and a computational framework for analysing multi-view, whole-CNS calcium imaging data. We image both brain and ventral nerve cord, covering the entire CNS at 2 or 5 Hz with two- or one-photon excitation, respectively. By mapping network activity during fictive behaviours and quantitatively comparing high-resolution whole-CNS activity maps across individuals, we predict functional connections between CNS regions and reveal neurons in the brain that identify type and temporal state of motor programs executed in the ventral nerve cord.

Suggested Citation

  • William C. Lemon & Stefan R. Pulver & Burkhard Höckendorf & Katie McDole & Kristin Branson & Jeremy Freeman & Philipp J. Keller, 2015. "Whole-central nervous system functional imaging in larval Drosophila," Nature Communications, Nature, vol. 6(1), pages 1-16, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8924
    DOI: 10.1038/ncomms8924
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    Cited by:

    1. Evan S. Schaffer & Neeli Mishra & Matthew R. Whiteway & Wenze Li & Michelle B. Vancura & Jason Freedman & Kripa B. Patel & Venkatakaushik Voleti & Liam Paninski & Elizabeth M. C. Hillman & L. F. Abbot, 2023. "The spatial and temporal structure of neural activity across the fly brain," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Sophie Aimon & Takeo Katsuki & Tongqiu Jia & Logan Grosenick & Michael Broxton & Karl Deisseroth & Terrence J Sejnowski & Ralph J Greenspan, 2019. "Fast near-whole–brain imaging in adult Drosophila during responses to stimuli and behavior," PLOS Biology, Public Library of Science, vol. 17(2), pages 1-31, February.
    3. Samuel K. H. Sy & Danny C. W. Chan & Roy C. H. Chan & Jing Lyu & Zhongqi Li & Kenneth K. Y. Wong & Chung Hang Jonathan Choi & Vincent C. T. Mok & Hei-Ming Lai & Owen Randlett & Yu Hu & Ho Ko, 2023. "An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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