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Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos

Author

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  • Thomas Jank

    (Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-University Freiburg)

  • Stephanie Eckerle

    (Institute Biology I, Faculty of Biology, Albert-Ludwigs-University Freiburg)

  • Marcus Steinemann

    (Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-University Freiburg)

  • Christoph Trillhaase

    (Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-University Freiburg)

  • Marianne Schimpl

    (MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee)

  • Sebastian Wiese

    (Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg)

  • Daan M. F. van Aalten

    (MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee)

  • Wolfgang Driever

    (Institute Biology I, Faculty of Biology, Albert-Ludwigs-University Freiburg
    Centre for Biological Signalling Studies (BIOSS), Albert-Ludwigs-University Freiburg)

  • Klaus Aktories

    (Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-University Freiburg
    Centre for Biological Signalling Studies (BIOSS), Albert-Ludwigs-University Freiburg)

Abstract

Yersinia species cause zoonotic infections, including enterocolitis and plague. Here we studied Yersinia ruckeri antifeeding prophage 18 (Afp18), the toxin component of the phage tail-derived protein translocation system Afp, which causes enteric redmouth disease in salmonid fish species. Here we show that microinjection of the glycosyltransferase domain Afp18G into zebrafish embryos blocks cytokinesis, actin-dependent motility and cell blebbing, eventually abrogating gastrulation. In zebrafish ZF4 cells, Afp18G depolymerizes actin stress fibres by mono-O-GlcNAcylation of RhoA at tyrosine-34; thereby Afp18G inhibits RhoA activation by guanine nucleotide exchange factors, and blocks RhoA, but not Rac and Cdc42 downstream signalling. The crystal structure of tyrosine-GlcNAcylated RhoA reveals an open conformation of the effector loop distinct from recently described structures of GDP- or GTP-bound RhoA. Unravelling of the molecular mechanism of the toxin component Afp18 as glycosyltransferase opens new perspectives in studies of phage tail-derived protein translocation systems, which are preserved from archaea to human pathogenic prokaryotes.

Suggested Citation

  • Thomas Jank & Stephanie Eckerle & Marcus Steinemann & Christoph Trillhaase & Marianne Schimpl & Sebastian Wiese & Daan M. F. van Aalten & Wolfgang Driever & Klaus Aktories, 2015. "Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos," Nature Communications, Nature, vol. 6(1), pages 1-16, November.
  • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8807
    DOI: 10.1038/ncomms8807
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    Cited by:

    1. Jaroslaw Sedzicki & Dongchun Ni & Frank Lehmann & Henning Stahlberg & Christoph Dehio, 2024. "Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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