IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8753.html
   My bibliography  Save this article

Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast

Author

Listed:
  • Atsushi Matsuda

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    Graduate School of Frontier Biosciences, Osaka University)

  • Yuji Chikashige

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology)

  • Da-Qiao Ding

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology)

  • Chizuru Ohtsuki

    (Graduate School of Frontier Biosciences, Osaka University)

  • Chie Mori

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology)

  • Haruhiko Asakawa

    (Graduate School of Frontier Biosciences, Osaka University)

  • Hiroshi Kimura

    (Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology)

  • Tokuko Haraguchi

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    Graduate School of Frontier Biosciences, Osaka University)

  • Yasushi Hiraoka

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    Graduate School of Frontier Biosciences, Osaka University)

Abstract

It is generally believed that silent chromatin is condensed and transcriptionally active chromatin is decondensed. However, little is known about the relationship between the condensation levels and gene expression. Here we report the condensation levels of interphase chromatin in the fission yeast Schizosaccharomyces pombe examined by super-resolution fluorescence microscopy. Unexpectedly, silent chromatin is less condensed than the euchromatin. Furthermore, the telomeric silent regions are flanked by highly condensed chromatin bodies, or ‘knobs’. Knob regions span ∼50 kb of sequence devoid of methylated histones. Knob condensation is independent of HP1 homologue Swi6 and other gene silencing factors. Disruption of methylation at lysine 36 of histone H3 (H3K36) eliminates knob formation and gene repression at the subtelomeric and adjacent knob regions. Thus, epigenetic marks at H3K36 play crucial roles in the formation of a unique chromatin structure and in gene regulation at those regions in S. pombe.

Suggested Citation

  • Atsushi Matsuda & Yuji Chikashige & Da-Qiao Ding & Chizuru Ohtsuki & Chie Mori & Haruhiko Asakawa & Hiroshi Kimura & Tokuko Haraguchi & Yasushi Hiraoka, 2015. "Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8753
    DOI: 10.1038/ncomms8753
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8753
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms8753?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Javier Encinar del Dedo & M. Belén Suárez & Rafael López-San Segundo & Alicia Vázquez-Bolado & Jingjing Sun & Natalia García-Blanco & Patricia García & Pauline Tricquet & Jun-Song Chen & Peter C. Dedo, 2024. "The Greatwall-Endosulfine-PP2A/B55 pathway regulates entry into quiescence by enhancing translation of Elongator-tunable transcripts," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8753. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.