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Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol

Author

Listed:
  • Moritz Hacke

    (Heidelberg University Biochemistry Center (BZH))

  • Patrik Björkholm

    (Cell and Molecular Biology, Science for Life Laboratory, Uppsala University)

  • Andrea Hellwig

    (Interdisciplinary Center for Neurosciences, Heidelberg University)

  • Patricia Himmels

    (Heidelberg University Biochemistry Center (BZH))

  • Carmen Ruiz de Almodóvar

    (Heidelberg University Biochemistry Center (BZH))

  • Britta Brügger

    (Heidelberg University Biochemistry Center (BZH))

  • Felix Wieland

    (Heidelberg University Biochemistry Center (BZH))

  • Andreas M. Ernst

    (Heidelberg University Biochemistry Center (BZH))

Abstract

The high pathogenicity of the Ebola virus reflects multiple concurrent processes on infection. Among other important determinants, Ebola fusogenic glycoprotein (GP) has been associated with the detachment of infected cells and eventually leads to vascular leakage and haemorrhagic fever. Here we report that the membrane-anchored GP is sufficient to induce the detachment of adherent cells. The results show that the detachment induced through either full-length GP1,2 or the subunit GP2 depends on cholesterol and the structure of the transmembrane domain. These data reveal a novel molecular mechanism in which GP regulates Ebola virus assembly and suggest that cholesterol-reducing agents could be useful as therapeutics to counteract GP-mediated cell detachment.

Suggested Citation

  • Moritz Hacke & Patrik Björkholm & Andrea Hellwig & Patricia Himmels & Carmen Ruiz de Almodóvar & Britta Brügger & Felix Wieland & Andreas M. Ernst, 2015. "Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol," Nature Communications, Nature, vol. 6(1), pages 1-9, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8688
    DOI: 10.1038/ncomms8688
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    Cited by:

    1. Jing Zhang & Bin Wang & Xiaoxiao Gao & Cheng Peng & Chao Shan & Silas F. Johnson & Richard C. Schwartz & Yong-Hui Zheng, 2022. "RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Sarah Goellner & Giray Enkavi & Vibhu Prasad & Solène Denolly & Sungmin Eu & Giulia Mizzon & Leander Witte & Waldemar Kulig & Zina M. Uckeley & Teresa M. Lavacca & Uta Haselmann & Pierre-Yves Lozach &, 2023. "Zika virus prM protein contains cholesterol binding motifs required for virus entry and assembly," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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