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Microsecond protein dynamics observed at the single-molecule level

Author

Listed:
  • Takuhiro Otosu

    (Molecular Spectroscopy Laboratory, RIKEN)

  • Kunihiko Ishii

    (Molecular Spectroscopy Laboratory, RIKEN
    Ultrafast Spectroscopy Research Team, RIKEN Center for Advanced Photonics (RAP), RIKEN)

  • Tahei Tahara

    (Molecular Spectroscopy Laboratory, RIKEN
    Ultrafast Spectroscopy Research Team, RIKEN Center for Advanced Photonics (RAP), RIKEN)

Abstract

How polypeptide chains acquire specific conformations to realize unique biological functions is a central problem of protein science. Single-molecule spectroscopy, combined with fluorescence resonance energy transfer, is utilized to study the conformational heterogeneity and the state-to-state transition dynamics of proteins on the submillisecond to second timescales. However, observation of the dynamics on the microsecond timescale is still very challenging. This timescale is important because the elementary processes of protein dynamics take place and direct comparison between experiment and simulation is possible. Here we report a new single-molecule technique to reveal the microsecond structural dynamics of proteins through correlation of the fluorescence lifetime. This method, two-dimensional fluorescence lifetime correlation spectroscopy, is applied to clarify the conformational dynamics of cytochrome c. Three conformational ensembles and the microsecond transitions in each ensemble are indicated from the correlation signal, demonstrating the importance of quantifying microsecond dynamics of proteins on the folding free energy landscape.

Suggested Citation

  • Takuhiro Otosu & Kunihiko Ishii & Tahei Tahara, 2015. "Microsecond protein dynamics observed at the single-molecule level," Nature Communications, Nature, vol. 6(1), pages 1-9, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8685
    DOI: 10.1038/ncomms8685
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    Cited by:

    1. Nicolas Couture & Wei Cui & Markus Lippl & Rachel Ostic & Défi Junior Jubgang Fandio & Eeswar Kumar Yalavarthi & Aswin Vishnuradhan & Angela Gamouras & Nicolas Y. Joly & Jean-Michel Ménard, 2023. "Single-pulse terahertz spectroscopy monitoring sub-millisecond time dynamics at a rate of 50 kHz," Nature Communications, Nature, vol. 14(1), pages 1-7, December.

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