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Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly

Author

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  • Gary Male

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1)

  • Alexander von Appen

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1)

  • Sebastian Glatt

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1)

  • Nicholas M. I. Taylor

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1
    Present address: Ecole Polytechnique Federale de Lausanne, BSP 416 (Cubotron UNIL), Route de la Sorge, CH-1015 Lausanne, Switzerland)

  • Michele Cristovao

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1)

  • Helga Groetsch

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1)

  • Martin Beck

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1)

  • Christoph W. Müller

    (European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1)

Abstract

In eukaryotes, RNA Polymerase III (Pol III) is specifically responsible for transcribing genes encoding tRNAs and other short non-coding RNAs. The recruitment of Pol III to tRNA-encoding genes requires the transcription factors (TF) IIIB and IIIC. TFIIIC has been described as a conserved, multi-subunit protein complex composed of two subcomplexes, called τA and τB. How these two subcomplexes are linked and how their interaction affects the formation of the Pol III pre-initiation complex (PIC) is poorly understood. Here we use chemical crosslinking mass spectrometry and determine the molecular architecture of TFIIIC. We further report the crystal structure of the essential TPR array from τA subunit τ131 and characterize its interaction with a central region of τB subunit τ138. The identified τ131–τ138 interacting region is essential in vivo and overlaps with TFIIIB-binding sites, revealing a crucial interaction platform for the regulation of tRNA transcription initiation.

Suggested Citation

  • Gary Male & Alexander von Appen & Sebastian Glatt & Nicholas M. I. Taylor & Michele Cristovao & Helga Groetsch & Martin Beck & Christoph W. Müller, 2015. "Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly," Nature Communications, Nature, vol. 6(1), pages 1-11, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8387
    DOI: 10.1038/ncomms8387
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    Cited by:

    1. Guillermo Abascal-Palacios & Laura Jochem & Carlos Pla-Prats & Fabienne Beuron & Alessandro Vannini, 2021. "Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes," Nature Communications, Nature, vol. 12(1), pages 1-11, December.

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