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CD24 tracks divergent pluripotent states in mouse and human cells

Author

Listed:
  • Nika Shakiba

    (Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto)

  • Carl A. White

    (Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
    The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto)

  • Yonatan Y. Lipsitz

    (Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto)

  • Ayako Yachie-Kinoshita

    (Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
    The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto)

  • Peter D Tonge

    (Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital)

  • Samer M. I. Hussein

    (Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital)

  • Mira C. Puri

    (Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
    University of Toronto)

  • Judith Elbaz

    (Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital)

  • James Morrissey-Scoot

    (Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto)

  • Mira Li

    (Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital)

  • Javier Munoz

    (Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University
    Padualaan 8
    Netherlands Proteomics Centre)

  • Marco Benevento

    (Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University
    Padualaan 8
    Netherlands Proteomics Centre)

  • Ian M. Rogers

    (Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
    University of Toronto
    University of Toronto)

  • Jacob H. Hanna

    (Weizmann Institute of Science)

  • Albert J. R. Heck

    (Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University
    Padualaan 8
    Netherlands Proteomics Centre)

  • Bernd Wollscheid

    (Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zürich
    NCCR Neuro Center for Proteomics, University and Swiss Federal Institute of Technology (ETH) Zürich
    Swiss Federal Institute of Technology (ETH) Zürich)

  • Andras Nagy

    (Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
    University of Toronto
    Institute of Medical Science, University of Toronto)

  • Peter W Zandstra

    (Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
    The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto)

Abstract

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

Suggested Citation

  • Nika Shakiba & Carl A. White & Yonatan Y. Lipsitz & Ayako Yachie-Kinoshita & Peter D Tonge & Samer M. I. Hussein & Mira C. Puri & Judith Elbaz & James Morrissey-Scoot & Mira Li & Javier Munoz & Marco , 2015. "CD24 tracks divergent pluripotent states in mouse and human cells," Nature Communications, Nature, vol. 6(1), pages 1-11, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8329
    DOI: 10.1038/ncomms8329
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