IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8316.html
   My bibliography  Save this article

A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells

Author

Listed:
  • Yuping Mei

    (University of Maryland School of Dentistry)

  • Yuyan Wang

    (University of Maryland School of Dentistry
    Peking University Cancer Hospital and Institute)

  • Priti Kumari

    (Institute for Genome Sciences, University of Maryland School of Medicine)

  • Amol Carl Shetty

    (Institute for Genome Sciences, University of Maryland School of Medicine)

  • David Clark

    (University of Maryland School of Dentistry)

  • Tyler Gable

    (University of Maryland School of Dentistry)

  • Alexander D. MacKerell

    (Computer-Aided Drug Design Center, University of Maryland School of Pharmacy
    Marlene and Stewart Greenebaum Cancer Center, University of Maryland)

  • Mark Z. Ma

    (University of Maryland School of Dentistry)

  • David J. Weber

    (Marlene and Stewart Greenebaum Cancer Center, University of Maryland
    University of Maryland School of Medicine)

  • Austin J. Yang

    (Marlene and Stewart Greenebaum Cancer Center, University of Maryland)

  • Martin J. Edelman

    (Marlene and Stewart Greenebaum Cancer Center, University of Maryland)

  • Li Mao

    (University of Maryland School of Dentistry
    Marlene and Stewart Greenebaum Cancer Center, University of Maryland)

Abstract

PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-like-163 (piR-L-163), the top downregulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM. The piR-L-163/p-ERM interaction is critical for p-ERM’s binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.

Suggested Citation

  • Yuping Mei & Yuyan Wang & Priti Kumari & Amol Carl Shetty & David Clark & Tyler Gable & Alexander D. MacKerell & Mark Z. Ma & David J. Weber & Austin J. Yang & Martin J. Edelman & Li Mao, 2015. "A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8316
    DOI: 10.1038/ncomms8316
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8316
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms8316?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8316. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.