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Structural insights into the translational infidelity mechanism

Author

Listed:
  • Alexey Rozov

    (Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université de Strasbourg; CNRS
    CNRS)

  • Natalia Demeshkina

    (Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université de Strasbourg; CNRS
    CNRS)

  • Eric Westhof

    (Architecture et Réactivité de l’ARN, Université de Strasbourg, Institut de Biologie Moléculaire et Cellulaire, CNRS)

  • Marat Yusupov

    (Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université de Strasbourg; CNRS
    CNRS)

  • Gulnara Yusupova

    (Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université de Strasbourg; CNRS
    CNRS)

Abstract

The decoding of mRNA on the ribosome is the least accurate process during genetic information transfer. Here we propose a unified decoding mechanism based on 11 high-resolution X-ray structures of the 70S ribosome that explains the occurrence of missense errors during translation. We determined ribosome structures in rare states where incorrect tRNAs were incorporated into the peptidyl-tRNA-binding site. These structures show that in the codon–anticodon duplex, a G·U mismatch adopts the Watson–Crick geometry, indicating a shift in the tautomeric equilibrium or ionization of the nucleobase. Additional structures with mismatches in the 70S decoding centre show that the binding of any tRNA induces identical rearrangements in the centre, which favours either isosteric or close to the Watson–Crick geometry codon–anticodon pairs. Overall, the results suggest that a mismatch escapes discrimination by preserving the shape of a Watson–Crick pair and indicate that geometric selection via tautomerism or ionization dominates the translational infidelity mechanism.

Suggested Citation

  • Alexey Rozov & Natalia Demeshkina & Eric Westhof & Marat Yusupov & Gulnara Yusupova, 2015. "Structural insights into the translational infidelity mechanism," Nature Communications, Nature, vol. 6(1), pages 1-9, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8251
    DOI: 10.1038/ncomms8251
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    Cited by:

    1. Juntaek Oh & Zelin Shan & Shuichi Hoshika & Jun Xu & Jenny Chong & Steven A. Benner & Dmitry Lyumkis & Dong Wang, 2023. "A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Dylan Girodat & Hans-Joachim Wieden & Scott C. Blanchard & Karissa Y. Sanbonmatsu, 2023. "Geometric alignment of aminoacyl-tRNA relative to catalytic centers of the ribosome underpins accurate mRNA decoding," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Joonas A. Jamsen & David D. Shock & Samuel H. Wilson, 2022. "Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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