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Single-molecule imaging of a three-component ordered actin disassembly mechanism

Author

Listed:
  • Silvia Jansen

    (Rosenstiel Basic Medical Science Research Center, Brandeis University)

  • Agnieszka Collins

    (Rosenstiel Basic Medical Science Research Center, Brandeis University)

  • Samantha M. Chin

    (Rosenstiel Basic Medical Science Research Center, Brandeis University)

  • Casey A. Ydenberg

    (Rosenstiel Basic Medical Science Research Center, Brandeis University)

  • Jeff Gelles

    (Brandeis University)

  • Bruce L. Goode

    (Rosenstiel Basic Medical Science Research Center, Brandeis University)

Abstract

The mechanisms by which cells destabilize and rapidly disassemble filamentous actin networks have remained elusive; however, Coronin, Cofilin and AIP1 have been implicated in this process. Here using multi-wavelength single-molecule fluorescence imaging, we show that mammalian Cor1B, Cof1 and AIP1 work in concert through a temporally ordered pathway to induce highly efficient severing and disassembly of actin filaments. Cor1B binds to filaments first, and dramatically accelerates the subsequent binding of Cof1, leading to heavily decorated, stabilized filaments. Cof1 in turn recruits AIP1, which rapidly triggers severing and remains bound to the newly generated barbed ends. New growth at barbed ends generated by severing was blocked specifically in the presence of all three proteins. This activity enabled us to reconstitute and directly visualize single actin filaments being rapidly polymerized by formins at their barbed ends while simultanteously being stochastically severed and capped along their lengths, and disassembled from their pointed ends.

Suggested Citation

  • Silvia Jansen & Agnieszka Collins & Samantha M. Chin & Casey A. Ydenberg & Jeff Gelles & Bruce L. Goode, 2015. "Single-molecule imaging of a three-component ordered actin disassembly mechanism," Nature Communications, Nature, vol. 6(1), pages 1-13, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8202
    DOI: 10.1038/ncomms8202
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    Cited by:

    1. Tommi Kotila & Hugo Wioland & Muniyandi Selvaraj & Konstantin Kogan & Lina Antenucci & Antoine Jégou & Juha T. Huiskonen & Guillaume Romet-Lemonne & Pekka Lappalainen, 2022. "Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Qianqian Ma & Wahyu Surya & Danxia He & Hanmeng Yang & Xiao Han & Mui Hoon Nai & Chwee Teck Lim & Jaume Torres & Yansong Miao, 2024. "Spa2 remodels ADP-actin via molecular condensation under glucose starvation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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