Author
Listed:
- Hideaki E. Kato
(Graduate School of Science, The University of Tokyo
Present address: Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305, USA)
- Motoshi Kamiya
(Graduate School of Science, Kyoto University)
- Seiya Sugo
(Graduate School of Science, Kyoto University)
- Jumpei Ito
(Graduate School of Bioagricultural Sciences, Nagoya University)
- Reiya Taniguchi
(Graduate School of Science, The University of Tokyo)
- Ayaka Orito
(Graduate School of Bioagricultural Sciences, Nagoya University)
- Kunio Hirata
(RIKEN SPring-8 Center)
- Ayumu Inutsuka
(Research Institute of Environmental Medicine, Nagoya University)
- Akihiro Yamanaka
(Research Institute of Environmental Medicine, Nagoya University)
- Andrés D. Maturana
(Graduate School of Bioagricultural Sciences, Nagoya University)
- Ryuichiro Ishitani
(Graduate School of Science, The University of Tokyo)
- Yuki Sudo
(Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University)
- Shigehiko Hayashi
(Graduate School of Science, Kyoto University)
- Osamu Nureki
(Graduate School of Science, The University of Tokyo)
Abstract
Microbial opsins with a bound chromophore function as photosensitive ion transporters and have been employed in optogenetics for the optical control of neuronal activity. Molecular engineering has been utilized to create colour variants for the functional augmentation of optogenetics tools, but was limited by the complexity of the protein–chromophore interactions. Here we report the development of blue-shifted colour variants by rational design at atomic resolution, achieved through accurate hybrid molecular simulations, electrophysiology and X-ray crystallography. The molecular simulation models and the crystal structure reveal the precisely designed conformational changes of the chromophore induced by combinatory mutations that shrink its π-conjugated system which, together with electrostatic tuning, produce large blue shifts of the absorption spectra by maximally 100 nm, while maintaining photosensitive ion transport activities. The design principle we elaborate is applicable to other microbial opsins, and clarifies the underlying molecular mechanism of the blue-shifted action spectra of microbial opsins recently isolated from natural sources.
Suggested Citation
Hideaki E. Kato & Motoshi Kamiya & Seiya Sugo & Jumpei Ito & Reiya Taniguchi & Ayaka Orito & Kunio Hirata & Ayumu Inutsuka & Akihiro Yamanaka & Andrés D. Maturana & Ryuichiro Ishitani & Yuki Sudo & Sh, 2015.
"Atomistic design of microbial opsin-based blue-shifted optogenetics tools,"
Nature Communications, Nature, vol. 6(1), pages 1-10, November.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8177
DOI: 10.1038/ncomms8177
Download full text from publisher
Citations
Citations are extracted by the
CitEc Project, subscribe to its
RSS feed for this item.
Cited by:
- Kyle Tucker & Savitha Sridharan & Hillel Adesnik & Stephen G. Brohawn, 2022.
"Cryo-EM structures of the channelrhodopsin ChRmine in lipid nanodiscs,"
Nature Communications, Nature, vol. 13(1), pages 1-12, December.
- R. Astashkin & K. Kovalev & S. Bukhdruker & S. Vaganova & A. Kuzmin & A. Alekseev & T. Balandin & D. Zabelskii & I. Gushchin & A. Royant & D. Volkov & G. Bourenkov & E. Koonin & M. Engelhard & E. Bamb, 2022.
"Structural insights into light-driven anion pumping in cyanobacteria,"
Nature Communications, Nature, vol. 13(1), pages 1-13, December.
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8177. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.